Specifications

Product Data
Applications	ICC/IF, IHC, IP, WB
Recommended Dilution	WB 1:500 - 1:2000;IF 1:50 - 1:200
Reactivities	Human, Mouse, Rat
Host	Rabbit
Isotype	IgG
Clonality	Polyclonal
Immunogen	Recombinant protein of human SOX2
Formulation	Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration	lot specific
Purification	Affinity purification
Conjugation	Unconjugated
Storage	Store at -20°C as received.
Stability	Stable for 12 months from date of receipt.
Predicted Protein Size	34 kDa
Gene Name	SRY-box 2
Database Link	NP_003097 Entrez Gene 20674 MouseEntrez Gene 499593 RatEntrez Gene 6657 Human UniProt ID P48431
Background	Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3), and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8).
Synonyms	ANOP3; MCOPS3
Reference Data
Protein Families	Adult stem cells, Cancer stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors