SYBR® Green Fluorescent DNA Stain
Referencia PCR-378-1ML
embalaje : 1ml
Marca : Jena Bioscience
Contact local distributor :
Teléfono : +1 850 650 7790
DNA intercalation dye for real-time PCR analysis
Excitation (blue) and emission (green) spectrum of SYBR® Green bound to dsDNA
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
store dark
Shelf Life: 12 months
Form: liquid, supplied in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20
Color: orange
Concentration: 100 μM
Spectroscopic Properties: λexc 495 nm, λem 520 nm (bound to DNA)
Description:
SYBR® Green Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.
SYBR® Green Fluorescent DNA Stain is supplied as 100 μM concentration. Vortex SYBR® Green Fluorescent DNA Stain thoroughly prior to its use. An SYBR® Green concentration of 0.5-1.0 μM in the final assay is recommended. Add SYBR® Green Fluorescent DNA Stain as indicated in the table below per assay. Please note that the preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors.
Select the optical setting for SYBR® Green or FAM on the detection instrument.
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.
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