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> TCP1-alpha Antibody

TCP1-alpha Antibody

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Referencia ASM10311-A565-100

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Marca : Abcepta

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  • ICC/IF - TCP1-alpha Antibody ASM10311
    Immunocytochemistry/Immunofluorescence analysis using Rat Anti-TCP1-alpha Monoclonal Antibody, Clone 91a (ASM10311). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody (ASM10311) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TCP1-alpha Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
  • WB - TCP1-alpha Antibody ASM10311
    Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of TCP1 alpha protein using Rat Anti-TCP1 alpha Monoclonal Antibody, Clone 91a (ASM10311). Primary Antibody: Rat Anti-TCP1 alpha Monoclonal Antibody (ASM10311) at 1:1000.
  • ICC/IF - TCP1-alpha Antibody ASM10311
    Immunocytochemistry/Immunofluorescence analysis using Rat Anti-TCP1-alpha Monoclonal Antibody, Clone 91a (ASM10311). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody (ASM10311) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-TCP1-alpha Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF, IP, FCM
Primary Accession P11983
Other Accession NP_038714.2
Host Rat
Isotype IgG2a
Reactivity Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Yeast, Bovine, C.Elegans, Guinea Pig, Dog, Drosophila
Clonality Monoclonal
Description Rat Anti-Mouse TCP1-alpha Monoclonal IgG2a
Target/Specificity Detects ~60kDa. Also detects ~92kDa. Cross reactivity with human HSP60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.
Other Names p63 Antibody, Tcp-1 Antibody, TCP1 alpha Antibody, Ccta Antibody, TRic Antibody, Tp63 Antibody, 21454 Antibody, c-cpn Antibody, CCT Antibody, Tcp1 Antibody, ccpn Antibody, AI528772 Antibody, Cct1 Antibody, Tcp 1 Antibody, c cpn Antibody
Clone Names 91a
Immunogen Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-479 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm