TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)

Referencia AH341-01

embalaje : 50rxns×20ul

Marca : TransGen Biotech

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Teléfono : +1 850 650 7790

TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)

Catalog Number: AH341-01

Price:Please inquire first

Specification:
50 rxns×20 µl system

Product Details

The kit provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds. The resulting cDNA is suitable for qPCR, not for regular PCR.

• Simultaneous genomic DNA removal and cDNA synthesis.
• The optimal ratio of Oligo(dT)20 Primer to random primer(N9) for qPCR ready cDNA.
• qPCR ready cDNA in 15 minutes.
• cDNA up to 250 bp.

Applications

• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

Storage

at -20 ℃ for two years

Shipping

Dry ice (-70 ℃)

Product Composition


Component   AH341-01 (50 rxns )
TransScript® II All-in-One SuperMix for qPCR                           200 μl
TransScript® II All-in-One No-RT Control SuperMix for qPCR                             20 μl
gDNA Remover                             50 μl
RNase-free Water                              1 ml


References

1 Rui C, Zhang Y, Fan Y, et al. Insight Between the Epigenetics and Transcription Responding of Cotton Hypocotyl Cellular Elongation Under Salt-Alkaline Stress[J]. Frontiers in Plant Science, 2021.(IF 5.73)

FAQ

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