Universal Tissue cDNA, Reverse Transcribed by Oligo dT Primer, Monkey Adult Normal, BioGenomics™

BioGenomics™ Tissue cDNA:
cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.

BioGenomics™ Universal cDNA
Mixed Male and Female donors. Prepared by reverse transcribed RNA by using random hexamer or oligo dT primer. Total RNA is isolated by modified guanidine thiocyanate techniques. The Universal cDNA serves as a standard for comparison of gene expression by real time PCR and regular PCR, and also as a gene pool for cloning genes.

Applications:
Suitable for use in SNP analysis, Southern Blot, PCR, Genomic DNA library construction, Profiling study in gene expression

Species Selection:
Choose from Human, Mouse, Rat, Chicken, Canine, Monkey.
• Human Universal cDNA is prepared from major organs of both male and female adult donors
• Mouse Universal cDNA is prepared from several male and female Balb/C mice whole bodies without fur.
• Rat Universal cDNA is prepared from several male and female Sprague Dawley or Wistar rats whole bodies without fur.
• Chicken (Leghorn) Universal cDNA is prepared from major organs of both male and female adult donors.
• Canine (Beagle) Universal cDNA is prepared from major organs of both male and female adult donors
• Monkey (Cynomolgus and Rhesus) Universal cDNA is prepared from major organs of both male and female adult donors

Supplied With:
T5595-0010: Tissue, cDNA, Actin Control Primer

Concentration:
2.5ng/ul; 1ul cDNA is sufficient for one PCR reaction.

Form:
Supplied as a liquid in 1X RT buffer: 50mM Tris-Cl, pH 8.3, 75mM KCl, 3mM MgCl2, 10mM DTT.

Control PCR Component:
Taq polymerase 0.2ul
10X PCR buffer 2.5ul
10mM dNTP 0.5ul
Control primers (5uM) 1.0ul
PCR Ready First Strand cDNA 1.0ul
Sterile ddH2O 19.8ul
Total Volume 25.0ul

Control PCR Conditions:
1. 94°C x 2 minutes, 1 cycle.
2. 94°C x 30 seconds, 55°C x 30 seconds, 72°C x 30 seconds, 35 cycles.
3. 72°C x 5 minutes, 1 cycle. Hold at 4°C.

Quality Control:
RNA Integrity for cDNA synthesis examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA using denaturing agarose gel.
Quality and purity of total RNA is determined spectrophotometrically.
A260/280 1.8 - 2.0 (10mM Tris-Cl, pH 7.5)
8S/18S Ratio ≥ 1
•. RNA used for cDNA synthesis is treated by DNase I and is tested as DNA-free RNA by PCR.
• The synthesized human, animal, and cell line cDNA was 5’ selected to ensure its full length.
• The cDNA was used as template for PCR amplification of beta-actin gene and an 838 bp beta-actin band was visualized on 1% agarose gel
• Synthesized plant cDNA was used as template for PCR amplification of chloroplast gene.
• 458bp chloroplast band visualized on 1% agarose gel.
β-actin control primer included, 10 PCR reactions
Chloroplast control primer included., 10 PCR reactions

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.