VE-Cadherin, phosphorylated (Y731) (Cadherin-5, 7B4 Antigen, Vascular Endothelial cadherin, VE-cadherin, CD144, CDH5, Cadherin 5)

The cadherins are a family of Ca 2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy-terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. VE-cadherin (for vascular endothelial cadherin, also designated cadherin-5) is localized at intercellular junctions of endothelial cells, where it is thought to play a role in the cohesion and organization of intercellular junctions.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Western Blot: 1:500-1:1000
Immunohistochemistry (Paraffin): 1:50-1:200
Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Pab

IgG

rabbit

human

~1mg/ml

Supplied as a liquid PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.

Purified by immunoaffinity chromatography.

Synthetic phosphopeptide derived from human VE-Cadherin around the phosphorylation site of Tyrosine 731.

Recognizes endogenous levels of human VE-Cadherin, phosphorylated Y731. Species Crossreactivity

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.