WGA Lectin (FITC)
Referencia GTX01502
embalaje : 1mg
Marca : Genetex
Contact local distributor :
Teléfono : +1 850 650 7790
Application | ICC/IF, IHC-P |
|---|---|
Species | Wheat |
| Package | 1 mg |
Datasheet File View product citations for antibody GTX01502 on CiteAb
PRODUCT
Summary
Wheat germ agglutinin (WGA) (MW 36 kDa) is a dimeric, carbohydrate-free protein composed of two identical subunits. The amino acid sequence provides a figure of 21,600, based on 171 amino acids per polypeptide chain. The protein dissociates into monomers in acidic media, pK of the reaction 4. The receptor sugar for WGA is N-acetyl glucosamine (GlcNAc), GlcNacβ14GlcNAc. These structures are present in many serum and membrane glycoproteins, bacterial cell wall peptidoglycans, chitin, cartilage glycosaminogyycons and glycolipids. Native WGA also react with glycoproteins with sialic acid residue. This lectin is useful for the purification of insulin receptors, serum proteins and neuronal tracing. The carbohydrate-binding specificity of WGA has been studied by variety of techniques, such as hapten inhibition of hemagglutination and specific precipitation of glycoconjugate, changes in fluorescence of the lectin (intrinsic) or of chromatogenic ligands (extrinsic), equilibrium dialysis, NMR and x-ray diffraction.
APPLICATION
Product Note
N-acetyl glucosamine (GlcNAc)
PROPERTIES
Form
Liquid
Buffer
10mM Phosphate (pH7.6), 150mM NaCl, 0.1mM Calcium Chloride
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
5 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
Native Protein
Expression System
Native Protein
Purification
Purified from Wheat Germ Agglutinin
Conjugation
Fluorescein isothiocyanate (FITC)
Absorption : 490 nm ; Emission : 520 nm. Ratio : 1.0-1.4 molecules FITC (isomer 1) per Lectin molecule.
Absorption : 490 nm ; Emission : 520 nm. Ratio : 1.0-1.4 molecules FITC (isomer 1) per Lectin molecule.
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Wheat Germ agglutinin , Triticum vulgaris , Wheat germ agglutinin lectin , Wheat germ agglutinin , Triticum vulgaris , GlcNAc lectin , WGA , WGA Lectin , Triticum vulgaris lectin
Background
Wheat germ agglutinin (WGA) (MW 36 kDa) is a dimeric, carbohydrate-free protein composed of two identical subunits. The amino acid sequence provides a figure of 21,600, based on 171 amino acids per polypeptide chain. The protein dissociates into monomers in acidic media, pK of the reaction 4. The receptor sugar for WGA is N-acetyl glucosamine (GlcNAc), GlcNacβ14GlcNAc. These structures are present in many serum and membrane glycoproteins, bacterial cell wall peptidoglycans, chitin, cartilage glycosaminogyycons and glycolipids. Native WGA also react with glycoproteins with sialic acid residue. This lectin is useful for the purification of insulin receptors, serum proteins and neuronal tracing. The carbohydrate-binding specificity of WGA has been studied by variety of techniques, such as hapten inhibition of hemagglutination and specific precipitation of glycoconjugate, changes in fluorescence of the lectin (intrinsic) or of chromatogenic ligands (extrinsic), equilibrium dialysis, NMR and x-ray diffraction. Inhibiting/Eluting sugars: 500 mM GlcNAc, dimer, trimmer or 100 mM acetic acid.Carbohydrate-Binding Specificity of WGA: Pentaacetylchitopentaose >Tetraacetylchitotetraose > triacetylchitotriose > Diacetylchitobiose > GlcNAc
Application Reference
G Wang et al. Front Pharmacol 2024; 15 Paucatalinone A from Paulownia Catalpifolia Gong Tong Elicits Mitochondrial-Mediated Cancer Cell Death to Combat Osteosarcoma
Application : IHC-P
Yan CY et al. iScience 2024; 27 (1) : 108690 Prenatal hormone stress triggers embryonic cardiac hypertrophy outcome by ubiquitin-dependent degradation of mitochondrial mitofusin 2.
Wang M et al. Theranostics 2023; 13 (7) : 2263-2280 OTUD1 promotes pathological cardiac remodeling and heart failure by targeting STAT3 in cardiomyocytes.
Shi J et al. STAR Protoc 2023; 4 (2) : 102172 Protocol for the identification and expression analysis of a cytoplasmic membrane-localized protein STING.
Crompton M et al. JCI Insight 2023; 8 (5) Mineralocorticoid receptor antagonism in diabetes reduces albuminuria by preserving the glomerular endothelial glycocalyx.
Submit a Reference REVIEW
Application Reference
G Wang et al. Front Pharmacol 2024; 15 Paucatalinone A from Paulownia Catalpifolia Gong Tong Elicits Mitochondrial-Mediated Cancer Cell Death to Combat Osteosarcoma
Application : IHC-P
Yan CY et al. iScience 2024; 27 (1):108690 Prenatal hormone stress triggers embryonic cardiac hypertrophy outcome by ubiquitin-dependent degradation of mitochondrial mitofusin 2.
Wang M et al. Theranostics 2023; 13 (7):2263-2280 OTUD1 promotes pathological cardiac remodeling and heart failure by targeting STAT3 in cardiomyocytes.
Shi J et al. STAR Protoc 2023; 4 (2):102172 Protocol for the identification and expression analysis of a cytoplasmic membrane-localized protein STING.
Crompton M et al. JCI Insight 2023; 8 (5) Mineralocorticoid receptor antagonism in diabetes reduces albuminuria by preserving the glomerular endothelial glycocalyx.
Grepper D et al. Mol Metab 2023; 68:101669 Methodological advancements in organ-specific ectopic lipid quantitative characterization: Effects of high fat diet on muscle and liver intracellular lipids.
Zhang X et al. Cell Cycle 2022; 21 (21):2309-2322 Astragaloside IV attenuate MI-induced myocardial fibrosis and cardiac remodeling by inhibiting ROS/caspase-1/GSDMD signaling pathway.
Zhengdong Gao et al. J Inflamm Res 2022; 15:3337-3353 Nociceptor Neurons are Involved in the Host Response to Escherichia coli Urinary Tract Infections.
Ding B et al. J Ethnopharmacol 2022; 291:115106 Centella asiatica (L.) Urb. attenuates cardiac hypertrophy and improves heart function through multi-level mechanisms revealed by systems pharmacology.
Li X et al. J Clin Invest 2021; (Epub) An alternatively spliced STING isoform localizes in the cytoplasmic membrane and directly senses extracellular cGAMP.
Zhang X et al. Evid Based Complement Alternat Med 2021; 2021:5518083 LuQi Formula Regulates NLRP3 Inflammasome to Relieve Myocardial-Infarction-Induced Cardiac Remodeling in Mice.
Zheng H et al. Front Cardiovasc Med 2021; 8:656093 circSnx12 Is Involved in Ferroptosis During Heart Failure by Targeting miR-224-5p.
Ye B et al. EMBO J 2020; 39 (13):e103786 The chromatin remodeler SRCAP promotes self-renewal of intestinal stem cells.
Hagan ML et al. Aging Cell 2019;:e13056 Decreased pericellular matrix production and selection for enhanced cell membrane repair may impair osteocyte responses to mechanical loading in the aging skeleton.
Huang TW et al. Acta Biomater 2019; (Epub) Regulation of chitosan-mediated differentiation of human olfactory receptor neurons by insulin-like growth factor binding protein-2.
Chen HR et al. PLoS Pathog 2018; 14 (4):e1007033 Macrophage migration inhibitory factor is critical for dengue NS1-induced endothelial glycocalyx degradation and hyperpermeability.
Zheng X et al. Exp Anim 2017; (Epub) Ginsenoside Rb1 improves cardiac function and remodeling in heart failure.
Zhang H et al. Chem Biol 2013; 20 (5):734-41 Selecting agonists from single cells infected with combinatorial antibody libraries.
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| Sodium Azide.pdf | |
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