WideUSE™ Plasmid Purification MiniSpin Kit
Referencia AN0068
embalaje : 50rxn
Marca : Canvax Biotech
WideUSE™ Plasmid Purification MiniSpin Kit
High-yield plasmid DNA isolation in ~30 minutes using WideUse™ silica MiniSpin technology.
The WideUse™ Plasmid Purification MiniSpin Kit provides a fast, reliable and cost-effective method for isolating high-quality plasmid DNA from E. coli. Based on advanced silica-membrane technology, the kit ensures reproducible yields of highly pure DNA free of proteins, RNA and other contaminants.
The extracted plasmid DNA is immediately ready for use in a wide range of downstream molecular biology applications, such as restriction digestion, PCR, qPCR, sequencing or transfection. Its optimized buffers and high-capacity columns guarantee consistent results even with challenging samples.
Designed for routine plasmid minipreps in research laboratories, the WideUse™ kit combines simplicity, reproducibility and superior performance at an affordable .
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range: 79.90 € through 259.90 €
Detailed information:
Advantages & Features
- Highly efficient: yields up to 24 µg (depending on plasmid copy number, culture volume and bacterial growth conditions).
- Broad range: optimized for plasmid sizes between 1 and 15 kb.
- Pure plasmid DNA: ready-to-use in all Molecular Biology applications.
- Swift and easy procedure: obtain plasmid DNA in ~25–30 minutes with minimal handling.
- Safe: avoids phenol/chloroform extraction.
- Convenient MiniSpin format: compatible with standard centrifuge
- Cost-effective solution for routine plasmid preps.
Specifications
Includes
Includes for 50 rxn:
– 50 CleanEasy™ MiniSpin Columns
– 50 Collection tubes
– 16 mL S-I Buffer
– 16 mL S-II Buffer
– 16 mL S-III Buffer
– 30 mL Binding Buffer
– 8 mL Washing Buffer
– 10 mL Elution Buffer
– 160 μL RNAse
Download documentation
Applications
Purified DNA suitable for all common Molecular Biology applications, such as:
- Restriction digestion and cloning
- Routine plasmid DNA preparation from E. coli
- Automated sequencing.
- PCR template.
- Transfection.
- Library construction & screening
Tables & Figures
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Quality Control
- Tested for the isolation of any plasmid DNA from transformed E.coli.
- The Quality of Purified DNA is analysed by:
- Ratio 260/ 280 (1.8-2).
- Agarose Gel Electrophoresis.
- Digestion with Restriction Endonucleases.
Advice
- Use fresh overnight cultures in exponential phase for maximum yields.
- Do not overload columns to ensure purity.
- Equilibrate buffers to room temperature before starting the procedure
Storage & Shipping
- Shipped in: Ambient Temperature.
- Storage: All components can be stored at Room Temperature (15–25 °C) , except RNAse A (-20 ºC).
- Recommendations: After addition of RNase A to Buffer S-I reagent can be stored at 4 ºC.
Citations
- Gómez-Melero, S. (2022). Desarrollo de anticuerpos terapéuticos contra el receptor CCR6 humano.
- Veva, A. M. M. (2021). Production of T. gondii transgenic strains to characterize the tubulin cofactor B interactome (Doctoral dissertation, Instituto Politécnico de Lisboa, Escola Superior de Tecnologia da Saúde de Lisboa).
- Jiménez Muñoz, R. (2020). Desarrollo de herramientas fisiológicas y genómicas para mejorar la calidad postcosecha del fruto de calabacín.
- Maza-Márquez, P., Aranda, E., González-López, J., & Rodelas, B. (2020). Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR). In Quantitative Real-Time PCR (pp. 79-94). Humana, New York, NY.
- Maza-Márquez, P., Castellano-Hinojosa, A., González-Martínez, A., Juárez-Jiménez, B., González-López, J., & Rodelas, B. (2019). Abundance of total and metabolically active Candidatus Microthrix and fungal populations in three full-scale wastewater treatment plants. Chemosphere.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
Plasmid miniprep kit, Plasmid DNA purification miniprep, Mini spin plasmid isolation kit, Plasmid extraction kit miniprep, Plasmid purification kit
Also known as:
- Spanish: Kit de purificación de plásmidos miniprep, Aislamiento de ADN plasmídico, Extracción de plásmidos
- French: Kit de purification plasmide miniprep, Isolation ADN plasmidique, Extraction plasmidique
- German: Plasmid-Miniprep-Kit, Plasmid-DNA-Isolation, Plasmidaufreinigung
- Italian: Kit purificazione plasmidi miniprep, Isolamento DNA plasmidico, Estrazione plasmidi