Anti-NKG2A,C,E (Biotin) Monoclonal Antibody

Referencia N2814-50B-500ug

embalaje : 500ug

Marca : US Biological

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N2814-50B NKG2A,C,E (Biotin)

Clone Type
Polyclonal
Host
rat
Source
mouse
Isotype
IgG2a,k
Grade
Purified
Applications
FC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

Mouse CD94/NKG2 is a natural killer (NK) cell receptor for the non-classical MHC class I molecule Qa-1b.2, 3 NKG2 is expressed on NK cells, lymphokine-activated killer (LAK) T cells, and some CD8+ memory T cells. Two-color immunofluorescent staining with the antibody reveals two distinct NKG2+ phenotypes within the NK1.1+CD3- spleen cell population: NKG2hi (50%) and NKG2low (50%). With the exception of DBA/2J mice, NKG2 is expressed on NK1.1+ cells from all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL).1

Applications:
• Flow cytometry 1

Recommended Dilution:
Flow Cytometry: ≤ 1ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Product Type: Mab|Isotype: IgG2a,k|Clone No: 20d5|Host: rat|Source: mouse|Concentration: ~0.5mg/ml|Form: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.|Purity: Purified|Immunogen: CHO cells transfected with murine NKG2|Specificity: Recognizes mouse NKG2 (Mr ~43kD).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
CHO cells transfected with murine NKG2
Form
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
Purity
Purified
Specificity
Recognizes mouse NKG2 (Mr ~43kD).
References
1. Vance, R.E. and D.H. Raulet. Personal communication.|2. Vance, R.E., J.R. Kraft, J.D. Altman, P.E. Jensen, and D.H. Raulet. 1998. J. Exp. Med. 188:1841.|3. Kraft, J.R., Vance, R.E., J. Pohl, A.M. Martin, D.H. Raulet, and Jensen, P.E. 2000. J. Exp. Med. 192:613.|4. Vance, R.E., A.M. Jamieson, and D.H. Raulet. 1999. J. Exp. Med. 190:1801.