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> Anti-OCT4 Antibody (4Q101)

Anti-OCT4 Antibody (4Q101)

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Referencia NB-64-118261-50uL

embalaje : 50uL

Marca : Neo Biotech


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Anti-OCT4 Antibody (4Q101)

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Catalog No. TMAC-02915 Copy Product Info
Anti-OCT4 Antibody (4Q101) is a Rabbit antibody targeting 45934. Anti-OCT4 Antibody (4Q101) can be used in ChIP,ICC/IF,IHC,IP,WB.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-OCT4 Antibody (4Q101) is a Rabbit antibody targeting 45934. Anti-OCT4 Antibody (4Q101) can be used in ChIP,ICC/IF,IHC,IP,WB.
Ig Type
IgG
Clone
4Q101
Reactivity
Human,Mouse
Verified Activity
1. Western blot analysis of Oct4 on different lysates using anti-Oct4 antibody at 1/1,000 dilution. Positive control: Lane 1: F9, Lane 2: NCCIT, Lane 3: hES.
2. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Oct4 antibody. Counter stained with hematoxylin.
3. ICC staining Oct4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining Oct4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Application
ChIP
ICC/IF
IHC
IP
WB
Recommended Dose
WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundPOU5F1 (POU domain, class 5, transcription factor 1), also known as octamer-binding transcription factor-3 (Oct-3, OTF3), octamer-binding transcription factor-4 (Oct-4, Otf-4) and Oct-3/4, modulates embryonic stem (ES) cell populations by influencing lineage commitment. Oct-3/4 sustains stem-cell self-renewal and differentiation pathways. Transcription factors containing the POU homeodomain regulate tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Oct-3/4 is capable of inducing rapid proliferation and tumorigenic properties of ES cells through activation of the UTF1 gene. In humans, two Oct-3/4 isoforms contribute to influencing the undifferentiated phenotype of ES cells. Oct-3/4 pseudogenes localizing to human chromosomes 10 and 8 are reported to be transcribed in certain cancer cell lines and tissues.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Q01860
Chemical Properties
Molecular WeightAntigen Theoretical: 39 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Keywords

transcription factor 1POU5F1POU5F 1POU domain, class 5, transcription factor 1OTF-3OTF3OTF 3Octamer-binding transcription factor 3 (OTF-3)Octamer-binding protein 4 (Oct-4)Octamer-binding protein 3 (Oct-3)class5class 54593445933
Related Tags: Anti-OCT4 Antibody (4Q101) molecular weight