Anti-SNAT1 Neurotransmitter Transporter Antibody (56522)
Referencia 56522-100
embalaje : 100ug
Marca : Leinco Technologies
AntiSNAT1 Neurotransmitter Transporter Antibody (56522)
AntiSNAT1 Neurotransmitter Transporter Antibody (56522)
Product No.: 56522
Clone S10432 Target SNAT1 Neurotransmitter Transporter Formats AvailableView All Product Type Monoclonal Alternate Names Amino acid transporter A1, rATA1, Glutamine transporter, Nsystem amino acid transporter 2, Solute carrier family 38 member 1, System A amino acid transporter 1, System A transporter 2, System N amino acid transporter 1 Isotype Mouse IgG1 Applications ICC , IF , IHC , WB |
Data
Western Blot analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse AntiSLC38A1 Monoclonal Antibody, Clone S10432 (56522). Primary Antibody: Mouse AntiSLC38A1 Monoclonal Antibody (56522) at 1:1000.
Immunocytochemistry/Immunofluorescence analysis using Mouse AntiSNAT1 Monoclonal Antibody, Clone S10432 (56522). Tissue: Neuroblastoma cells (SHSY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse AntiSNAT1 Monoclonal Antibody (56522) at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: PhalloidiniFluor 647 (red) FActin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) PhalloidiniFluor 647 (red) FActin stain. (C) SNAT1 Antibody (D) Composite.
Immunocytochemistry/Immunofluorescence analysis using Mouse AntiSNAT1 Monoclonal Antibody, Clone S10432 (56522). Tissue: Neuroblastoma cell line (SKNBE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse AntiSNAT1 Monoclonal Antibody (56522) at 1:100 for 60 min at RT. Secondary Antibody: Goat AntiMouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red FActin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red FActin stain. (C) SNAT1 Antibody. (D) Composite.Antibody DetailsProduct DetailsReactive Species Human ⋅ Mouse ⋅ Rat Host Species Mouse Immunogen Fusion protein corresponding to aa 163 (cytoplasmic Nterminus) of rat SNAT1 (accession no. NP_620187.1). Product Concentration 1.0 mg/ml Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This product is stable for at least 1 year at 20°C. Freeze in multiple aliquots to avoid repeated freezethaw cycles. Regulatory Status For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 28°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 12ug/mL. A band of ~50kDa is detected. Immunohistochemistry: use at 15ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Rat neocortical neurons cultured under amino acid starvation conditions. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes human, mouse, and rat SNAT1. Background The sodiumcoupled neutral amino acid transporters (SNAT) include System A subtypes SNAT1, SNAT2, and SNAT4 and System N subtypes SNAT3 and SNAT5. These transporters are essential for uptake of nutrients, energy production, metabolism, detoxification, and the cycling of neurotransmitters. SNAT1 is responsible for transport of glutamine and may be involved in the generation of glutamate in the retina. SNAT1 is found in heart, brain, and placenta, and expression levels are high in certain neuronal populations in the central nervous system. Function Functions as a sodiumdependent amino acid transporter. Mediates the saturable, pHsensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA. {PubMed:10660562, PubMed:11692272, PubMed:11756489, PubMed:12684517}. NCBI Gene Bank ID UniProt.org Research Area Neuroscience References & Citations |