Reactivos e instrumentos para inmunología, biología celular y biología molecular.

AURION R-Gent SE-LM

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Referencia 500.011

embalaje : 60ml

Contact local distributor :

Teléfono : +1 850 650 7790

Marca : Aurion

Contact local distributor :

Teléfono : +1 850 650 7790

Datasheet (RG)

The Immunogold Silver Staining technique (IGSS) finds application both at the electron microscope and the light microscope level. In addition the technique is used to identify proteins or nucleic acids after blotting.

Light microscopical and macroscopical visualization of the latent gold signal requires an enhancement system that renders a high contrast signal. Light insensitivity and negligible autonucleation are required for ease of handling and low background. AURION R-Gent SE-LM is a two-component reagent that meets these requirements.

Introduction
The silver enhancement reaction is based on the gold particle catalyzed reduction of Ag+ to metallic silver using photographic developing compounds as the electron source. For light microscopy and immunoblotting applications the generated silver signal should be of high contrast. Furthermore the signal should be permanent and compatible with counterstaining.

Product Description
The AURION R-GENT SE-LM components constitute a Silver Enhancement Reagent which increases the average gold cluster or particle size by deposition of metallic silver facilitating detection at the light microscopical level. The generated brown-black signal is also easily detected in bio assays and is compatible with counterstaining in light microscopy. AURION R-GENT SE-LM has been tailored for the enhancement of AURION Ultra Small Immunogold reagents and is equally suited for the larger sized particles in the AURION Conventional Immunogold reagents.

For enhancement, equal amounts of the DEVELOPER and ENHANCER are mixed well and applied to the specimen. The enhancement mixture is easy-to-use, exhibits extremely delayed auto-nucleation and can be used under standard laboratory light conditions. Typical enhancement times are between 15 and 25 minutes. Auto-nucleation becomes visible only after 40 minutes. Light microscopical specimens may be counterstained according to standard procedures. The enhancement mixture has a pH value of 8.2-8.6.

AURION R-GENT SE-LM is available as a kit in two unit sizes (2 x 30 ml or 2 x 250 ml) and consists of a separate DEVELOPER and ENHANCER. The supplied amounts accomodate 600 and 5000 LM specimens respectively at 100 µl/specimen, or 60 and 500 bio assay specimens at 1 ml/specimen. The reactivity is tested on dot-spots and the absence of autonucleation is monitored by spectrophotometric techniques.

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