CD45 / LCA Rat Monoclonal Antibody [Clone ID: EM-05]

Specifications

Product Data
Clone Name	EM-05
Applications	FC
Recommended Dilution	Flow cytometry: 4 µg/ml as starting point.
Reactivities	Mouse
Host	Rat
Isotype	IgG
Clonality	Monoclonal
Immunogen	Murine peripheral blood leukocytes
Specificity	The antibody reacts with CD45 antigen (Leukocyte Common Antigen), a single chain type I transmembrane protein expressed at high level on cells of hematopoietic origin, except erythrocytes and platelets.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 Label: PerCP State: Liquid Ig fraction Label: Conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Concentration	lot specific
Conjugation	PerCP
Storage	Store undiluted at 2-8°C. DO NOT FREEZE! This products is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Gene Name	protein tyrosine phosphatase, receptor type, C
Database Link	Entrez Gene 19264 Mouse UniProt ID P06800
Background	CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data