Orb1821219-100ml | ELISA Wash Buffer, 10X Clinisciences

ELISA Wash Buffer, 10X

SKU: orb1821219

Description

ELISA Wash Buffer effectively rinses ELISA plates between reagent addition steps to remove signal-altering debris yet preserve assay components to reduce background and increase signal. Suitable for antibody-sandwich ELISAs and antigen-down ELISAs.

Images & Validation

−

Application Notes	1. Dilute ELISA Wash Buffer, 10X by adding 1 part ELISA Wash Buffer to 9 parts deionized water and mix for 15 minutes. ELISA Wash Buffer, 10X is supplied concentrated, so crystalline precipitates may form in the bottle, especially when refrigerated. If this happens, gently warm or mix the buffer until all crystals are dissolved. Do not let it boil. 2. Completely fill the wells of the ELISA plate with 1X wash buffer (about 400 µL/well). The diluted wash buffer may be dispensed through a squirt bottle, a plate washer, through a multi-channel pipette, or through an automated system. 3. Aspirate or dump out the buffer and repeat for a total of 2-4 washes. 4. After the final dump or aspiration, pound the plate on paper towels to remove any excess liquid. If washing the plate during the coating process, we recommend 2-3 washes after the coating step before adding the block buffer. If washing the plate as part of an ELISA procedure, we recommend 3-4 washes after the sample incubation and after the conjugate incubation.

Application Notes

1. Dilute ELISA Wash Buffer, 10X by adding 1 part ELISA Wash Buffer to 9 parts deionized water and mix for 15 minutes. ELISA Wash Buffer, 10X is supplied concentrated, so crystalline precipitates may form in the bottle, especially when refrigerated. If this happens, gently warm or mix the buffer until all crystals are dissolved. Do not let it boil. 2. Completely fill the wells of the ELISA plate with 1X wash buffer (about 400 µL/well). The diluted wash buffer may be dispensed through a squirt bottle, a plate washer, through a multi-channel pipette, or through an automated system. 3. Aspirate or dump out the buffer and repeat for a total of 2-4 washes. 4. After the final dump or aspiration, pound the plate on paper towels to remove any excess liquid. If washing the plate during the coating process, we recommend 2-3 washes after the coating step before adding the block buffer. If washing the plate as part of an ELISA procedure, we recommend 3-4 washes after the sample incubation and after the conjugate incubation.

Key Properties

−

Storage & Handling

−

Storage	Room temp
Concentration	10 X
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

−

10x Elisa Wash Buffer [orb436746]
Sizes Available: 100 ml
10x Elisa Wash Buffer [orb436747]
Sizes Available: 500 ml
10x Elisa Wash Buffer [orb436748]
Sizes Available: 1000 ml
ELISA Wash Buffer, 10X [orb623170]
Sizes Available: 1 l
ELISA Wash Buffer, 10X [orb1821218]
Sizes Available: 500 ml

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

ELISA Wash Buffer, 10X (orb1821219)

Based on 0 reviews

Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.

No reviews yet

ELISA Wash Buffer, 10X

Referencia orb1821219-100ml

Marca : Biorbyt

ELISA Wash Buffer, 10X

Description

Images & Validation

Key Properties

Storage & Handling

10x Elisa Wash Buffer [orb436746]

10x Elisa Wash Buffer [orb436747]

10x Elisa Wash Buffer [orb436748]

ELISA Wash Buffer, 10X [orb623170]

ELISA Wash Buffer, 10X [orb1821218]

Documents Download

Request a Document

Protocol Information

ELISA Wash Buffer, 10X (orb1821219)