Formaldehyde Detection Kit
Referencia OKAU00001
embalaje : 2plate
Marca : Aviva Systems Biology
Formaldehyde Detection Kit (OKAU00001)
| Datasheets/Manuals | Printable datasheet for Formaldehyde Detection Kit (OKAU00001) |
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| Application | ELISA-Sandwich | ||||||||||||||||||||||||||||||
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| ELISA Kit Linearity | Linearity was determined by taking two human urine samples, one with a low formaldehyde level of 17.6 uM and one with a higher level of 79.9 uM, and mixing them in the ratios given below. The measured concentrations were compared to the expected values.
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| ELISA Kit Principle | The DetectX® Formaldehyde Kit is designed to quantitatively measure formaldehyde present in tissue culture media and urine samples. Please read the complete kit insert before performing this assay. A formaldehyde standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a black microtiter plate. The fluorescent reaction is initiated with the DetectX® Formaldehyde reagent, which is pipetted into each well. After a short incubation the emission of the generated fluorescent signal is detected in a microtiter plate reader capable of measuring 510 nm fluorescence utilizing 450 nm excitation wavelength. The concentration of the formaldehyde in the sample is calculated, after making a suitable correction for the dilution of the sample, using software available with most fluorescence plate readers. | ||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra Assay Precision Four human urine samples were diluted 1:4 with deionized water and run in replicates of 20 in an assay. The mean and precision of the calculated formaldehyde concentrations were:
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| ELISA Kit Component |
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| Additional Information | Background: Formaldehyde (methanal), H2C=O, is a colorless, flammable, strong-smelling gas. It is an important industrial chemical used to manufacture building materials and to produce many household products. In the US approximately 3 x 10^9 kg are produced annually. In addition, formaldehyde is commonly used as an industrial fungicide, germicide, and disinfectant, and as a preservative in mortuaries and medical laboratories. Materials containing formaldehyde can release formaldehyde gas or vapor into the air. Formaldehyde can also be released by burning wood, kerosene, natural gas, or cigarettes, from automobile emissions, and from natural processes. Formaldehyde can undergo rapid chemical changes immediately after absorption. Studies have suggested that formaldehyde may affect the lymphatic and blood systems and that exposure to formaldehyde may cause leukemia, particularly myeloid leukemia, in humans. Industrial workers who help to produce formaldehyde or formaldehyde-containing products, laboratory technicians, health care professionals, and mortuary employees may be exposed to higher levels of formaldehyde than the general public. Exposure occurs primarily by inhaling formaldehyde gas or vapor from the air or by absorbing liquids containing formaldehyde through the skin. The National Cancer Institute (NCI) has determined that there is an association between occupational exposure to formaldehyde and an increase in the risk of cancer. Several NCI studies have found that anatomists and embalmers, professions with potential exposure to formaldehyde, are at an increased risk for leukemia and brain cancer compared with the general population. For example a multi-centered US study determined increased risk of nasopharyngeal cancer with formaldehyde exposure. | ||||||||||||||||||||||||||||||
| :: | Cross Reactivity: A variety of aldehydes, ketones and inorganic compounds were tested for their ability to give a false reading in the assay. These were made up at 0.1M (equal to 100,000 uM), diluted to 100 uM and tested in the assay. The following cross reactivities were observed.
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| Reconstitution and Storage | 2°C to 8°C | ||||||||||||||||||||||||||||||
| Sample Type | Human Urine and Tissue Culture Media | ||||||||||||||||||||||||||||||
| Sensitivity | 0.715 uM |
| Gene Full Name | Formaldehyde |
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