Glutamic Acid Decarboxylase 65, Recombinant Mouse, His-Tag, Western Blot Control (GAD65, GAD2)

Gamma Aminobutyric acid (GABA) is the major known inhibitory neurotransmitter. The rate-limiting step in the synthesis of GABA is the decarboxylation of glutamate by glutamate decarboxylase (GAD; L-glutamate 1-carboxy-lyase, EC 4.1.115). In the CNS GAB is entirely restricted to GABAergic neurons. GAD is also present in the b-cells of the pancreas and autoantibodies to various GAD polypeptides are detected in insulin-dependent diabetes mellitus. Cloning of GAD genes have identified two subtypes: GAD65 (65kD; human 585aa chromosome 10) and GAD67 (67kD; human 594aa, chromosome 2) share approx. 65% amino acid homology. The N-terminus is the most divergent while the C-terminus is highly conserved. Although both GAD isoforms catalyzes the conversion of GABA but interact differently with the co-factor pyridoxal 5'-phosphate suggesting their activities are differentially regulated. GAD67 is cytosolic, while GAD65 is membrane associated. GAD65 is a major autoantigen in diabetes mellitus and stiff-man syndrome, a rare disease of the brain.

Source:
Recombinant protein corresponding to full length mouse GAD65, fused to His-tag, expressed in E. coli.

Molecular Weight:
~67kD

Applications:
Suitable for use in ELISA, Western Blot as control, Immunohistochemistry as negative control. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.