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  • Line colors represent the approximate visible colors of the wavelength maxima.
  • Immunofluorescent analysis of beta Tubulin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST. The secondary antibody (red) was Goat Anti-Mouse IgG (H&L) - AF594 in PBS at room temperature in the dark.
Product NameGoat Anti-Mouse IgG (H&L) - AF594
Cat No: CSA3608
Source: Goat
Reactivity: M
Applications: E, IH, IF, FC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Datasheet
MSDS
Description: Goat Polyclonal Secondary Antibody to Mouse IgG (H&L) - AF594
Immunogen: Mouse IgG
Purification: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Clonality: Polyclonal
Conjugation: AF594
Form: Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide
Dilution: IH (1/100 - 1/1000), IF (1/100 - 1/1000), FC (1/1000 - 1/4000), E (Use at an assay dependent concentration)
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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AF594" ["liaison"]=> string(18) "Secondary antibody" ["espece"]=> string(4) "goat" ["couplage"]=> string(14) "AbFluor™ 594" ["application"]=> string(11) "IHC, IF, FC" ["clone"]=> string(10) "Polyclonal" ["cible"]=> string(29) "Goat Mouse IgG (H&L) - AF594" ["temperature"]=> string(5) "+4°C" ["site_country"]=> string(41) "0,1,5,6,11,25,27,34,36,41,47,51,54,58,219" ["quantity_min_order"]=> int(2) ["unspsc"]=> string(8) "12352203" ["importance"]=> int(99) ["eclass_5_1_4"]=> string(8) "32160702" ["eclass_10_0_1"]=> string(8) "32160702" ["eclass_6_1"]=> string(8) "32160702" ["eclass_12"]=> string(8) "32160702" ["hacat_name"]=> string(6) "RD2002" ["hacat_code"]=> string(6) "RD2002" } } CSA3608-100ul | Goat Polyclonal Secondary Antibody to Mouse IgG (H)
Reactivos e instrumentos para inmunología, biología celular y biología molecular.
 
> Goat Polyclonal Secondary Antibody to Mouse IgG (H&L) - AF594

Goat Polyclonal Secondary Antibody to Mouse IgG (H&L) - AF594

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Referencia CSA3608-100ul

embalaje : 100ul

Marca : Cohesion Biosciences

Contact local distributor :


Teléfono : +1 850 650 7790

  • Line colors represent the approximate visible colors of the wavelength maxima.
  • Immunofluorescent analysis of beta Tubulin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST. The secondary antibody (red) was Goat Anti-Mouse IgG (H&L) - AF594 in PBS at room temperature in the dark.
Product NameGoat Anti-Mouse IgG (H&L) - AF594
Cat No: CSA3608
Source: Goat
Reactivity: M
Applications: E, IH, IF, FC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Datasheet
Description: Goat Polyclonal Secondary Antibody to Mouse IgG (H&L) - AF594
Immunogen: Mouse IgG
Purification: The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Clonality: Polyclonal
Conjugation: AF594
Form: Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide
Dilution: IH (1/100 - 1/1000), IF (1/100 - 1/1000), FC (1/1000 - 1/4000), E (Use at an assay dependent concentration)
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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Referencia
Descripción
Cond.
Precio Sin IVA
4030-05
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 1.0mL