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> HIER Tris-EDTA Buffer pH 8,0(10x), for 5.000 ml

HIER Tris-EDTA Buffer pH 8,0(10x), for 5.000 ml

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Referencia orb1087599-1U

embalaje : 1unit

Marca : Biorbyt


HIER Tris-EDTA Buffer pH 8,0(10x), for 5.000 ml

SKU: orb1087599

Description

500 ml HIER EDTA Buffer pH 8.0 (10fold concentrated, adequate for 5 litres ready-to-use EDTA Buffer)

Research Area

Metabolism Research

Images & Validation

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HIER Tris-EDTA Buffer pH 8,0(10x), for 5.000 ml
Application Notes
HIER EDTA Buffer pH 8.0 is a 10fold concentrated, buffered EDTA solution with additives of detergent and stabilising substances. For preparation of the working strength solution the buffer concentrate is diluted 1:10 with deionised or distilled water. The resulting solution has a pH of 8.0 (7.8 to 8.2). HIER EDTA Buffer pH 8.0 is a very efficient epitope retrieval solution in immunohistochemical staining procedures to be used with primary antibodies of different specificity. HIER EDTA Buffer pH 8.0 leads to considerably stronger signals compared with usually used citrate buffer.

Key Properties

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ImmunogenPlease contact us for more information.

Storage & Handling

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StorageThe solution should be stored at 2-8°C. Do not free ze it. Under these conditions the solution is stable up to the expiry date indicated on the label. Do not use product after the expiry date. If stored at room temperature the solution is stable for at least 10 month from the date of delivery. The prepared working strength solution is stable for 1 month, if stored at 2-8°C. A positive and a negative control have to be carried out in parallel to the test material. If you observe unusual staining or other deviations from the expected results which could possibly be caused by this reagent, please contact our technical support.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Protocol Information

IHC-P
Immunohistochemistry Paraffin
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IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
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