Human N-Cadherin Recombinant Protein (C-Fc)

Referencia orb3149310-100ug

embalaje : 100ug

Marca : Biorbyt


Human N-Cadherin Recombinant Protein (C-Fc)

SKU: orb3149310

Description

Recombinant Human CD325/CDH2 Protein, C-Fc is produced in Mammalian Cells and supplied in lyophilized form. The protein includes the regions of Met1-Ala724 and carries the C-Fc tag. With a molecular weight of 105.98 kDa, it is suitable for ELISA, SDS-PAGE and WB applications. This product has a purity of greater than 95% as determined by SDS-PAGE.

Research Area

Immunology & Inflammation

Images & Validation

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Tested ApplicationsELISA, SDS-PAGE, WB

Key Properties

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Expression SystemMammalian cells
Biological OriginHomo sapiens (Human)
Expression RegionMet1-Ala724
MW105.98 kDa
Purity>95% as determined by SDS-PAGE.

Storage & Handling

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StorageUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Form/AppearanceLyophilized
Buffer/PreservativesLyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
ReconstitutionReconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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Cadherin-2, CDw325, Neural cadherin, N-cadherin, CD325, CDH2, CDHN, NCAD
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UniProt Details

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No UniProt data available

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol