Reactivos e instrumentos para inmunología, biología celular y biología molecular.

IgM (FITC)

Imprimir

Referencia I1905-01F-1mg

embalaje : 1mg

Marca : US Biological

Solicitar más información

I1905-01F IgM (FITC)

Clone Type

Polyclonal

Host

goat

Source

human

Isotype

IgG

Grade

Affinity Purified

Applications

FLISA IF WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications:
Suitable for use in FLISA, Western Blot and Immunofluorescence. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Absorption Wavelength:
495nm

Emission Wavelength:
528nm

Fluorochrome/Protein Ratio:
6.6 moles FITC per mole of Goat IgG

Storage and Stability:
Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications

Product Type: Pab|Isotype: IgG|Host: goat|Source: human|Concentration: ~1mg/ml (after reconstitution) |Form: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with Fluorescein isothiocyanate (FITC). |Purity: Purified by immunoaffinity chromatography.|Immunogen: Human IgM mu chain|Specificity: Recognizes human IgM (mu chain). Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, human IgM and human serum. No reaction was observed against human IgG or human IgA. Specificity was confirmed by ELISA at < 1% crossreactivity against other human heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Human IgM mu chain

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with Fluorescein isothiocyanate (FITC).

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human IgM (mu chain). Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, human IgM and human serum. No reaction was observed against human IgG or human IgA. Specificity was confirmed by ELISA at < 1% crossreactivity against other human heavy or light chain isotypes.