MDH1 ELISA Kit (Rat)
Referencia OKCD02635
embalaje : 96Wells
Marca : Aviva Systems Biology
MDH1 ELISA Kit (Rat) (OKCD02635)
| Datasheets/Manuals | Printable datasheet for MDH1 ELISA Kit (Rat) (OKCD02635) |
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| Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Malate Dehydrogenase 1 (MDH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Malate Dehydrogenase 1 (MDH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Malate Dehydrogenase 1 (MDH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Malate Dehydrogenase 1 (MDH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Malate Dehydrogenase 1 (MDH1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Malate Dehydrogenase 1 (MDH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | -20°C|2°C to 8°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.072 ng/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Malate Dehydrogenase 1 (MDH1). No significant cross-reactivity or interference between Malate Dehydrogenase 1 (MDH1) and analogues was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
| Protocol Information |
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| Gene Symbol | Mdh1 |
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| Gene Full Name | malate dehydrogenase 1 |
| Alias Symbols | cytosolic malate dehydrogenase, malate dehydrogenase 1, NAD (soluble), Malate dehydrogenase-like enzyme, malate dehydrogenase, cytoplasmic, malate dehydrogenase, peroxisomal, malate dehydrogenase, soluble, Mdhl, MDL1, Mor2. |
| NCBI Gene Id | 24551 |
| Protein Name | Malate dehydrogenase, cytoplasmic |
| Uniprot ID | O88989 |
| Protein Accession # | NP_150238.1 |
| Nucleotide Accession # | NM_033235.2 |
| Protein Size (# AA) | 334 |
