Quantibody® Mouse Cytokine Array 4000 Kit. A combination of Mouse Cytokine Array Q4, Mouse Cytokine Array Q5, Mouse Cytokine Array Q6, Mouse Cytokine Array Q7, and Mouse Cytokine Array Q8. Detects 200 Mouse Cytokines. Suitable for all liquid sample types.
High Density
Mouse Cytokine Array Q4000
Mouse Cytokine Array Q4000
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 200 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 250 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| 4-1BB (TNFRSF9/CD137)
| 6Ckine (CCL21)
| Ace
| Activin A
| ADAMTS-1 (METH1)
|
| Adiponectin (ACRP30)
| ALK-1
| Amphiregulin
| ANGPTL1
| ANGPTL3
|
| Artemin
| Axl
| BAFF R
| Betacellulin (BTC)
| bFGF
|
| BLC (CXCL13)
| C5a
| Cardiotrophin-1 (CT-1)
| CCL28 (MEC)
| CCL6
|
| CD27 (TNFRSF7)
| CD27 Ligand (TNFSF7)
| CD30 (TNFRSF8)
| CD30 Ligand (TNFSF8)
| CD32a
|
| CD36 (SR-B3)
| CD40 (TNFRSF5)
| CD40 Ligand (TNFSF5)
| CD48 (SLAMF2)
| CD6
|
| CD80 (B7-1)
| Chemerin
| Chordin
| Clusterin
| CRP (C-Reactive Protein)
|
| CTLA-4 (CD152)
| CXCL16
| Cystatin C
| DAN
| Decorin
|
| DKK-1
| DLL4
| Dtk
| E-Cadherin
| EDAR
|
| EGF
| Endocan
| Endoglin (CD105)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
|
| Epigen
| Epiregulin
| E-Selectin
| Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
|
| Fetuin A
| Flt-3 Ligand
| Fractalkine (CX3CL1)
| Galectin-1
| Galectin-3
|
| Galectin-7
| Gas 1
| Gas 6
| GCSF
| GITR (TNFRSF18)
|
| GITR Ligand (TNFSF18)
| GM-CSF
| gp130
| Granzyme B
| Gremlin-1
|
| H60
| HAI-1
| HGF
| HGFR
| I-309 (TCA-3/CCL1)
|
Species Detected
Mouse
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Biomarker Screening
- Identifying Key Factors
- Confirming Biological Process
- Biomarker Validation
- Validation of Antibody Array Results
- Quantitative Protein Detection
- Establishing Normal Range
Kit Components
- Mouse Cytokine Array Q4000 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAM-CYT-4.gal)
GAL (GAL-QAM-CYT-5.gal)
GAL (GAL-QAM-CYT-6.gal)
GAL (GAL-QAM-CYT-7.gal)
GAL (GAL-QAM-CYT-8.gal)
Excel (QAM-CYT-4_Analysis_Tool.xls)
Excel (QAM-CYT-5_Analysis_Tool.xls)
Excel (QAM-CYT-6_Analysis_Tool.xls)
Excel (QAM-CYT-7_Analysis_Tool.xls)
Excel (QAM-CYT-8_Analysis_Tool.xls)
citations
8-oxo-7,8-dihydroguanosine (8-oxo-Guo) drives pulmonary inflammatory pathways through pattern recognition receptors
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- 2026
- Journal Name:
- Redox Biology
- PubMed ID:
- 42190561
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- 2025
- Journal Name:
- Nature Communications
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- 40610476
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- Blood Advances
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LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexia
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Journal of Hematology & Oncology
A BMP-2–triggered in vivo osteo-organoid for cell therapy
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- Mouse
- Sample Type:
- femur
- Year:
- 2023
- Journal Name:
- Science advances
Secretory MPP3 reinforce myeloid differentiation trajectory and amplify myeloid cell production
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- Mouse
- Sample Type:
- BM fluid (Hematopoietic stem cells (HSC))
- Year:
- 2023
- Journal Name:
- Journal of Experimental Medicine
VEGF-A in serum protects against memory impairment in APP/PS1 transgenic mice by blocking neutrophil infiltration
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Molecular Psychiatry
Multi-omic analysis of the cardiac cellulome defines a vascular contribution to cardiac diastolic dysfunction in obese female mice
- Species:
- Mouse
- Sample Type:
- left ventricular
- Year:
- 2023
- Journal Name:
- Basic Research in Cardiology