Multiplex ELISA Kit For Human MMP (6-plex)
Referencia MEK1023
embalaje : 1kit
Marca : Boster Bio
Product Info Summary
| Catalog: | MEK1023 |
|---|---|
| Species: | Human |
| Sample Type: | Serum, not recommended for plasma |
| Volume Required per Well: | Min 25 µl |
| Assay Length: | 5.5 hours |
| Specificity: | All analytes < 1% intra-panel cross-reactivity |
| Assay Type: | Planar Based Multiplexed ELISA |
| Detection Method: | Chemiluminescent |
| Multiplex Format: | 96-well solid plate |
| Manufacturer Reference Number: | 340949HU |
| Analytes: | MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13 |
Product Overview
The Multiplex Human MMP (6-plex) is a fully quantitative ELISA-based chemiluminescent assay. This kit allows for the concurrent measurement of six matrix metalloproteinases (MMPs). MMPs are an important enzyme in cell biology, involved in cleaving extracellular matrix and cell surface receptor proteins (such as cytokines, chemokines, and apoptotic ligands). The specific markers/analytes included in this kit are MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, and MMP-13.
Using just 25 µl of sample per well, up to 80 samples can be assayed for six MMPs in approximately 5.5 hours. Multiplex Arrays provide researchers with an easy-to-use and cost-effective means of generating an MMP profile for each sample.
Multiplex plates are built by adsorbing multiple distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Assay Ranges
| Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection | Precision (Inter-assay) | Precision (Intra-assay) | Average Linearity |
|---|---|---|---|---|---|---|---|---|
| MMP-1 | Sandwich | 12,000 – 49.38 (pg/mL) | 8,400 (pg/mL) | 64.2 (pg/mL) | 24.7 (pg/mL) | 12% | 6% | 87% |
| MMP-2 | Sandwich | 80,000 - 329 (pg/mL) | 56,000 (pg/mL) | 428 (pg/mL) | 165 (pg/mL) | 11% | 9% | 105% |
| MMP-3 | Sandwich | 4,500 - 18.52 (pg/mL) | 3,150 (pg/mL) | 24.1 (pg/mL) | 9.26 (pg/mL) | 9% | 6% | 97% |
| MMP-7 | Sandwich | 1,800 - 7.41 (pg/mL) | 1,260 (pg/mL) | 9.6 (pg/mL) | 3.7 (pg/mL) | 14% | 11% | 98% |
| MMP-9 | Sandwich | 44,000 - 181 (pg/mL) | 30,800 (pg/mL) | 235.4 (pg/mL) | 90.53 (pg/mL) | 7% | 7% | 136% |
| MMP-13 | Sandwich | 35,000 - 144 (pg/mL) | 24,500 (pg/mL) | 187.2 (pg/mL) | 72.02 (pg/mL) | 13% | 7% | 100% |
Kit Components
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Assay Diluent: Liquid, 10 mL/vial of a buffered protein solution with preservatives
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips