RayBio® C-Series Rat Cytokine Antibody Array 1 Kit. Detects 19 Rat Cytokines. Suitable for all liquid sample types.
Rat Cytokine Array C1
Rat Cytokine Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Rat |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 19 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
CINC-2
| CINC-3
| CNTF
| Fractalkine (CX3CL1)
| GM-CSF
|
IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-4
| IL-6
|
IL-10
| LIX
| Leptin
| MCP-1 (CCL2)
| MIP-3 alpha (CCL20)
|
beta-NGF
| TIMP-1
| TNF alpha
| VEGF-A
| |
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Rat Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Celastrol nanomicelles attenuate cytokine secretion in macrophages and inhibit macrophage-induced corneal neovascularization in rats
- Species:
- Rat
- Sample Type:
- macrophages
- Year:
- 2023
- Journal Name:
- International Journal of Nanomedicine
Inhibition of retinal ischemia–reperfusion injury in rats by inhalation of low-concentration hydrogen gas
- Species:
- Rat
- Sample Type:
- retinal tissue
- Year:
- 2023
- Journal Name:
- Graefes Archive for Clinical and Experimental Ophthalmology
Gx-50 Inhibits Neuroinflammation via 7 nAChR Activation of the JAK2/STAT3 and PI3K/AKT Pathways
- Species:
- Mouse
- Sample Type:
- Conditioned Media (BV-2 microglial cells)
- Year:
- 2016
- Journal Name:
- Journal of Alzheimers Disease
- PubMed ID:
- 26836188
Urolithins at physiological concentrations affect the levels of pro-inflammatory cytokines and growth factor in cultured cardiac cells in hyperglucidic conditions
- Species:
- Rat
- Sample Type:
- Conditioned Media (Cardiomyocytes in culture)
- Year:
- 2015
- Journal Name:
- Journal of Functional Foods
Release of endogenous heat shock protein 72 on the survival of sepsis in rats
- Species:
- Rat
- Sample Type:
- Serum (Septic and Control Rats)
- Year:
- 2015
- Journal Name:
- Journal of Surgical Research
- PubMed ID:
- 26073348
Efficient differentiation of neural stem cells induced by the rat bone marrow stromal cells
- Species:
- Rat
- Sample Type:
- Conditioned Media (BMSCs cultures)
- Year:
- 2015
- Journal Name:
- International Journal of Clinical and Experimental Medicine
- PubMed ID:
- 26221209
Dichloroacetate prevents restenosis in preclinical animal models of vessel injury
- Species:
- Rat
- Sample Type:
- Tissue Lysate (Rat Injured Aortas)
- Year:
- 2014
- Journal Name:
- Nature
- PubMed ID:
- 24747400
Blocking lymphocyte trafficking with FTY720 prevents inflammation-sensitized hypoxic-ischemic brain injury in newborns
- Species:
- Rat
- Sample Type:
- Tissue Lysate (Rat Brain Tissue)
- Year:
- 2014
- Journal Name:
- Journal of Neuroscience
- PubMed ID:
- 25471584
Secretion of matrix metalloproteinase-9 from astrocytes by inhibition of tonic P2Y14-receptor-mediated signal (s).
- Species:
- Rat
- Sample Type:
- Conditioned Media (astrocytes)
- Year:
- 2013
- Journal Name:
- Cellular and Molecular Neurobiology
- PubMed ID:
- 22872320
Effects of EdU labeling on mesenchymal stem cells.
- Species:
- Rat
- Sample Type:
- Conditioned Media (EdUlabeled or unlabeled ADSC)
- Year:
- 2013
- Journal Name:
- Cytotherapy
- PubMed ID:
- 23260086