Rat Cytokine Array GS67

Referencia GSR-CAA-67-2

embalaje : 32SampleKit

Marca : RayBiotech


Rat Cytokine Array GS67

G-Series Rat Cytokine Array 67 Kit. A combination of 2 non-overlapping arrays to measure the relative expression levels of 67 rat cytokines. Suitable for all liquid sample types.
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Product Description

Specifications

Size16 Sample Kit, 32 Sample Kit, 64 Sample Kit
SpeciesRat
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeSemi-Quantitative
Number of Targets Detected67
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Shipping TypeBlue ice
Storage-20°C

Introduction

RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).

Product Features

  • High sensitivity (pg/mL to ng/mL levels)
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Test 16 samples on each slide
  • Data analysis software provides one-step computation

Target Names

Scroll over each target protein for more information
4-1BB
(TNFRSF9/CD137)
  • Gene Names: TNFRSF9
  • Accession #: Q4W8J3
Activin A
  • Gene Names: INHBA
  • Gene ID: 29200
  • Accession #: P18331
  • ELISA Kit: RayBio Rat Activin-A ELISA
Adiponectin
(ACRP30)
  • Gene Names: ADIPOQ ACDC ACRP30 APM1 GBP28
  • Gene ID: 246253;
  • Accession #: Q8K3R4
beta-NGF
  • Gene Names: NGF NGFB
  • Accession #: Q06225
  • ELISA Kit: RayBio Rat beta-NGF ELISA
CD48
(SLAMF2)
  • Gene Names: CD48 BCM1 BLAST1
  • Gene ID: 245962
  • Accession #: P10252
  • ELISA Kit: RayBio Rat CD48 ELISA
CD80
(B7-1)
  • Gene Names: CD80 CD28LG CD28LG1 LAB7
  • Accession #: Q62680
CD86
(B7-2)
  • Gene Names: CD86 CD28LG2
  • Gene ID: 56822
  • Accession #: O35531
  • ELISA Kit: RayBio Rat CD86 ELISA
CINC-1
(CXCL1)
  • Gene Names: Cxcl1 Cinc1 Gro Scyb1
  • Gene ID: 81503
  • Accession #: P14095
  • ELISA Kit: RayBio Rat CINC-1 ELISA
CINC-2
  • Gene Names: Cxcl3 Cinc2
  • Gene ID: 171551
  • Accession #: Q10746
  • ELISA Kit: RayBio Rat CINC-2 ELISA
CINC-3
  • Gene Names: Cxcl2 Cinc3 Mip-2 Mip2 Scyb2
  • Gene ID: 114105
  • Accession #: P30348
  • ELISA Kit: RayBio Rat CINC-3 ELISA
CNTF
  • Gene Names: CNTF
  • Gene ID: 25707
  • Accession #: P20294
  • ELISA Kit: RayBio Rat CNTF ELISA
CTACK
(CCL27)
  • Gene Names: CCL27 ILC SCYA27
  • Gene ID: 362505
  • Accession #: D4AAL6
Decorin
  • Gene Names: DCN
  • Gene ID: 29139
  • Accession #: Q01129
  • ELISA Kit: RayBio Rat Decorin ELISA
Eotaxin-1
(CCL11)
  • Gene Names: CCL11 SCYA11
  • Gene ID: 29397
  • Accession #: P97545
  • ELISA Kit: RayBio Rat Eotaxin-1 ELISA
EphA5
  • Gene Names: Epha5 Ehk-1 Ekh1 Rek7
  • Gene ID: 79208
  • Accession #: P54757
Erythropoietin
  • Gene Names: EPOR
  • Gene ID: 24335
  • Accession #: P29676
FGF-BP
  • Gene Names: FGFBP1 FGFBP HBP17
  • Gene ID: 64535
  • Accession #: Q9QY10
Flt-3 Ligand
  • Gene Names: FLT3LG
  • ELISA Kit: RayBio Rat Flt-3 Ligand ELISA
Fractalkine
(CX3CL1)
  • Gene Names: CX3CL1 FKN NTT SCYD1 A-152E5.2
  • Accession #: Q6IRF7
  • ELISA Kit: RayBio Rat Fractalkine ELISA
Galectin-1
  • Gene Names: LGALS1
  • Gene ID: 56646
  • Accession #: P11762
  • ELISA Kit: RayBio Rat Galectin-1 ELISA
Galectin-3
  • Gene Names: LGALS3
  • Gene ID: 83781
  • Accession #: P08699
  • ELISA Kit: RayBio Rat Galectin-3 ELISA
Gas 1
  • Gene Names: GAS1
  • Gene ID: 683470
  • Accession #: M0RBH9
  • ELISA Kit: RayBio Rat Gas 1 ELISA
GFR alpha-1
(GDNF R)
  • Gene Names: Gfra1 Gdnfra Retl1 Trnr1
  • Gene ID: 25454
  • Accession #: Q62997
GM-CSF
  • Gene Names: CSF2
  • Gene ID: 116630
  • Accession #: P48750
  • ELISA Kit: RayBio Rat GM-CSF ELISA
gp130
  • Gene Names: IL6ST
  • Accession #: P40190
HGF
  • Gene Names: MET
  • Gene ID: 24446
  • Accession #: P17945
  • ELISA Kit: RayBio Rat HGF ELISA
ICAM-1
(CD54)
  • Gene Names: ICAM1
  • Gene ID: 25464
  • Accession #: Q00238
  • ELISA Kit: RayBio Rat ICAM-1 ELISA
IFN-gamma
  • Gene Names: IFNG
  • Gene ID: 25712
  • Accession #: P01581
  • ELISA Kit: RayBio Rat IFN-gamma ELISA
IL-1 alpha
(IL-1 F1)
  • Gene Names: IL1A
  • Gene ID: 24493
  • Accession #: P16598
  • ELISA Kit: RayBio Rat IL-1 alpha ELISA
IL-1 beta
(IL-1 F2)
  • Gene Names: IL1B
  • Accession #: Q63264
  • ELISA Kit: RayBio Rat IL-1 beta ELISA
IL-1 R6
(IL-1 Rrp2)
  • Gene Names: IL1RL2 IL1RRP2
  • Gene ID: 171106
  • Accession #: Q62929
  • ELISA Kit: RayBio Rat IL-1 R6 ELISA
IL-1 Ra
(IL-1 F3)
  • Gene Names: IL1RN IL1RA
  • Gene ID: 60582
  • Accession #: P25086
  • ELISA Kit: RayBio Rat IL-1 Ra ELISA
IL-2
  • Gene Names: IL2 IL-2
  • Gene ID: 116562
  • Accession #: P17108
  • ELISA Kit: RayBio Rat IL-2 ELISA
IL-2 R alpha
  • Gene Names: IL2RA
  • Gene ID: 25704
  • Accession #: P26897
IL-3
  • Gene Names: IL3
  • Accession #: P04823
IL-4
  • Gene Names: IL4
  • Gene ID: 287287
  • Accession #: P20096
  • ELISA Kit: RayBio Rat IL-4 ELISA
IL-6
  • Gene Names: IL6
  • Gene ID: 24498
  • Accession #: P20607
  • ELISA Kit: RayBio Rat IL-6 ELISA
IL-7
  • Gene Names: IL7
  • Accession #: P56478
IL-10
  • Gene Names: IL10
  • Gene ID: 25325
  • Accession #: P29456
  • ELISA Kit: RayBio Rat IL-10 ELISA
IL-13
  • Gene Names: IL13
  • Gene ID: 116553
  • Accession #: P42203
  • ELISA Kit: RayBio Rat IL-13 ELISA
    (For Lysates)
IL-17F
  • Gene Names: IL17F
  • Gene ID: 301291
  • Accession #: Q5BJ95
IL-22
  • Gene Names: IL22 ILTIF ZCYTO18 UNQ3099/PRO10096
  • Gene ID: 500836
  • Accession #: G3V6X6
JAM-A
(CD321/F11R)
  • Gene Names: F11R JAM1 JCAM UNQ264/PRO301
  • Gene ID: 116479
  • Accession #: Q9JHY1
  • ELISA Kit: RayBio Rat JAM-A ELISA
LIX
  • Gene Names: Cxcl5 Scyb5
  • Gene ID: 60665
  • Accession #: P97885
  • ELISA Kit: RayBio Rat LIX ELISA
L-Selectin
(CD62L)
  • Gene Names: SELL LNHR LYAM1
  • Accession #: P30836
  • ELISA Kit: RayBio Rat L-Selectin ELISA
MCP-1
(CCL2)
  • Gene Names: CCL2 SCYA2
  • Gene ID: 24770
  • Accession #: P14844
  • ELISA Kit: RayBio Rat MCP-1 ELISA
MIP-1 alpha
(CCL3)
  • Gene Names: CCL3
  • Gene ID: 25542
  • Accession #: P50229
Neuropilin-1
  • Gene Names: Nrp1
  • Gene ID: 246331
  • Accession #: Q9QWJ9
Neuropilin-2
  • Gene Names: Nrp2
  • Gene ID: 81527
  • Accession #: O35276
Nope
  • Accession #: B5DFA9
  • ELISA Kit: RayBio Rat Nope ELISA
Notch-1
  • Gene Names: NOTCH1 TAN1
  • Gene ID: 25496
  • Accession #: Q07008
Notch-2
  • Gene Names: Notch2
  • Gene ID: 29492
  • Accession #: Q9QW30
P-Cadherin
  • Gene Names: CDH3 CDHP
  • Accession #: Q9JIV6
  • ELISA Kit: RayBio Rat P-Cadherin ELISA
PDGF-AA
  • Gene Names: PDGFA PDGF1
  • Gene ID: 25266
  • Accession #: Q6P7C3
  • ELISA Kit: RayBio Rat PDGF-AA ELISA
Prolactin
  • Gene Names: PRL
  • Gene ID: 24683
  • Accession #: P01237
  • ELISA Kit: RayBio Rat Prolactin ELISA
Prolactin R
  • Gene Names: Prlr
  • Gene ID: 24684
  • Accession #: P05710
  • ELISA Kit: RayBio Rat Prolactin R ELISA
RAGE
  • Accession #: Q63495
  • ELISA Kit: RayBio Rat RAGE ELISA
RANTES
(CCL5)
  • Gene Names: CCL5 SCYA5
  • Accession #: P50231
  • ELISA Kit: RayBio Rat RANTES ELISA
SCF
  • Gene Names: KITLG SCF
  • Gene ID: 60427
  • Accession #: P21581
TCK-1
(CXCL7)
  • Gene Names: Ppbp Cxcl7 mCG_1702
  • Gene ID: 246358
  • Accession #: Q99ME0
  • ELISA Kit: RayBio Rat TCK-1 ELISA
TIM-1
(KIM-1)
  • Gene Names: HAVCR1 KIM1 TIM1 TIMD1
  • Gene ID: 286934
  • Accession #: O54947
  • ELISA Kit: RayBio Rat TIM-1 ELISA
TIMP-1
  • Gene Names: TIMP1 CLGI TIMP
  • Gene ID: 116510
  • Accession #: P30120
  • ELISA Kit: RayBio Rat TIMP-1 ELISA
TIMP-2
  • Gene Names: TIMP2
  • Gene ID: 29543
  • Accession #: P30121
  • ELISA Kit: RayBio Rat TIMP-2 ELISA
TNF alpha
  • Gene Names: TNF TNFA TNFSF2
  • Gene ID: 103694380; 24835
  • Accession #: P16599
  • ELISA Kit: RayBio Rat TNF-alpha ELISA
TREM-1
  • Gene Names: TREM1
  • Gene ID: 301229;
  • Accession #: D4ABU7
  • ELISA Kit: RayBio Rat TREM-1 ELISA
TWEAK R
(TNFRSF12)
  • Gene ID: 302965;
  • Accession #: Q80XX9
  • ELISA Kit: RayBio Rat TWEAK R ELISA
VEGF-A
  • Gene Names: VEGFA VEGF
  • Gene ID: 83785
  • Accession #: P16612
  • ELISA Kit: RayBio Rat VEGF-A ELISA

Species Detected

Rat

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Detection of Relative Protein Expression
  • Detecting Patterns of Cytokine Expression
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming a Biological Process
Kit Components
  • Antibody Array Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktails
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

GAL (GAL-QAR-CYT-3.gal)
GAL (GAL-QAR-CYT-4.gal)
Excel (GSR-CYT-3_Analysis_Tool.xlsx)
Excel (GSR-CYT-4_Analysis_Tool.xlsx)

citations

  • Switching On and Off Macrophages by a “BridgeBurning” Coating Improves BoneImplant Integration under Osteoporosis

    Species:
    Rat
    Sample Type:
    Conditioned media (Cell culture media)
    Year:
    2020
  • Duloxetine protected indomethacin-induced gastric mucosal injury by increasing serotonin-dependent RANTES expression and activating PI3K-AKT-VEGF pathway

    Year:
    2024
    Journal Name:
    Toxicology and Applied Pharmacology
    PubMed ID:
    38701902
  • Water Rewarming After Seawater Hypothermia Mitigates IL-1β in Both Intestinal Tissue and Blood

    Species:
    Rat
    Sample Type:
    intestinal tissue
    Year:
    2023
    Journal Name:
    Therapeutic Hypothermia and Temperature Management