Rat N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT
Referencia CSB-E08752r-96T
embalaje : 96T
Marca : Cusabio
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
N-terminal pro-brain natriuretic peptide,NT-proBNP
Abbreviation
NT-proBNP
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-105 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
Recovery
| The recovery of rat NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 94 | 89-99 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Rat NT-proBNP ELISA Kit was designed for the quantitative measurement of Rat NT-proBNP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .
Citations
- B Martín-Carro, S Fernández-Villabrille,International Journal of Molecular Sciences,2025
- HJ Yuan, QC Han, YD Yu, H Yu,International Journal of Molecular Medicine,2025
- R Parvan, N Rolim, AB Gevaert, A Cataliotti,ESC Heart Failure,2025
- L Li, S Zhong, J Ye, S Hu, X Xiong,BMC Complementary Medicine and Therapies,2025
- TS Aldayel, OE Kilany, HNG El-Hak, HMA Abdelrazek,Life,2024
- Y Zhang, Z Shen, Z Mao, D Huang, C Lou,Frontiers in Bioscience-Landmark,2024
- L Abou Assi,Pharmaceutics,2024
- D Chen,European journal of pharmacology,2023
- B Martín-Carro,International Journal of Molecular Sciences,2023
- S Sun,International journal of molecular sciences,2022
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