Flow Cytometry
Flow cytometric analysis of MCF-7 cells using RET polyclonal antibody (Cat # PAB3381)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot (Cell lysate)
Western blot analysis of MCF-7 cell lysate (35 ug/lane) with RET polyclonal antibody (Cat # PAB3381).
- Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RET.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human RET.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Flow Cytometry (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using RET polyclonal antibody (Cat # PAB3381)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Western Blot (Cell lysate)
Western blot analysis of MCF-7 cell lysate (35 ug/lane) with RET polyclonal antibody (Cat # PAB3381). - Gene Info — RET
Entrez GeneID
5979Protein Accession#
NP_066124;P07949Gene Name
RET
Gene Alias
CDHF12, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET-ELE1, RET51
Gene Description
ret proto-oncogene
Gene Ontology
HyperlinkGene Summary
This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq
Other Designations
RET transforming sequence|cadherin family member 12|hydroxyaryl-protein kinase|oncogene RET|receptor tyrosine kinase|ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease)
- Interactomes
- Pathways
- Diseases
- Publication Reference
- A novel germ-line point mutation in RET exon 8 (Gly(533)Cys) in a large kindred with familial medullary thyroid carcinoma.
Da Silva AM, Maciel RM, Da Silva MR, Toledo SR, De Carvalho MB, Cerutti JM.
The Journal of Clinical Endocrinology and Metabolism 2003 Nov; 88(11):5438.
- Intronic single nucleotide polymorphisms in the RET protooncogene are associated with a subset of apparently sporadic pheochromocytoma and may modulate age of onset.
McWhinney SR, Boru G, Binkley PK, Peczkowska M, Januszewicz AA, Neumann HP, Eng C.
The Journal of Clinical Endocrinology and Metabolism 2003 Oct; 88(10):4911.
- The tyrosine phosphatase Shp-2 mediates intracellular signaling initiated by Ret mutants.
D'Alessio A, Califano D, Incoronato M, Santelli G, Florio T, Schettini G, Carlomagno MS, Cerchia L, de Franciscis V.
Endocrinology 2003 Oct; 144(10):4298.
Application：IP, WB-Tr, Monkey, Rat, COS-7, PC-12 cells.
- A novel germ-line point mutation in RET exon 8 (Gly(533)Cys) in a large kindred with familial medullary thyroid carcinoma.