RM FGF-BASIC (AF)
Referencia 200-12AF-50UG
embalaje : 50UG
Marca : FUJIFILM
Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis. FGF-basic also has cardioprotective functions during heart injury. The application of FGF-basic is a critical component for human embryonic stem cell culture systems and is necessary for maintaining human embryonic stem cells in an undifferentiated state.
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SDS-PAGE Showing a Single Prominent Band at ~ 16.5 kDa
Figure 1. rm FGF-Basic (1μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Instant Blue staining showing a single prominent band at ~ 18 kDa
Cell-Based Proliferation Assay
Figure 2. Recombinant Mouse FGF-basic / FGF-2 induced proliferation in NR6R/3T3 cells.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | FGF2, HBGF-2, Prostatropin |
| Accession Number | P15655 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Monomer, 16.5 kDa (146 aa) |
| AA Sequence | MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5 |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | 3T3 cell proliferation | ≤ 2.5 ng/mL |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.