Reactivos e instrumentos para inmunología, biología celular y biología molecular.

TCF 1 (T-cell Factor 1) Blocking Peptide

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Referencia T2020-04-100ul

embalaje : 100ul

Marca : US Biological

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T2020-04 TCF 1 (T-cell Factor 1) Blocking Peptide

Grade

Highly Purified

Shipping Temp

Blue Ice

Storage Temp

-20°C

Peptide is used to block TCF1 (C63D9) Rabbit mAb reactivity in immunohistochemistry protocols.

LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors which consists of the following: Lymphoid enhancer factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3 and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1/TCF bind to Wnt response elements to provide a docking site for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1/TCF proteins are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5).

Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks TCF1 (C63D9) Rabbit mAb by immunohistochemistry.

Directions for Use: For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100ul total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet.

Storage and Stability:
For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications

Purity: Highly Purified|Form: Supplied as a liquid in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Form

Supplied as a liquid in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol.

Purity

Highly Purified

References

(1) Waterman, M.L. (2004) Cancer Metastasis Rev. 23, 41–52. |(2) Schilham, M.W. and Clevers, H. (1998) Semin. Immunol. 10, 127–132. |(3) Brantjes, H. et al. (2002) Biol. Chem. 383, 255–261. |(4) Reya, T. and Clevers, H. (2005) Nature 434, 843–850. |(5) Logan, C.Y. and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781–810.