AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
Cell Line Validation
To validate the quality of our AAV control particles, we have developed the following procedure:
AAV genome titer is determined by qPCR.
AAV transduction efficiency is analyzed by in vitro transduction in HEK293T.
Shipping
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Description
AAV6 with CMV promoter-driven expression of GFP, >10^13 GC/mL, 50 ul
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.