Alpha Synuclein Antibody (pSer129): ATTO 488
Referencia MBS8000789-5x0,1mL
embalaje : 5x0.1mL
Marca : MyBiosource
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody (SPC-742). Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 ug/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody (SPC-742) at 1:500 for 24 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.
Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa Alpha Synuclein pSer129 protein using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody (SPC-742). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody (SPC-742) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.
Alpha Synuclein (SNCA) Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8000789Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally,… More details
Product Overview
Online Datasheet in PDFProduct Name
Alpha Synuclein (SNCA), Polyclonal Antibody
Full Product Name
Alpha Synuclein Antibody (pSer129): ATTO 488
Product Gene Name
anti-SNCA antibody
Product Synonym Names
Rabbit Anti-Human Alpha Synuclein pSer129 Polyclonal; Phospho anti-alpha Synuclein (S129) antibody; Alpha-synuclein (phospho S129) antibody; alpha Synuclein (phospho Ser129) antibody; alpha-Synuclein Phospho Ser129 Antibody; phospho-alpha-Synuclein (Ser129) Antibody; Alpha synuclein antibody; Alpha synuclein antibody phospho Serine 129; Alpha synuclein antibody phospho Ser 129; Alpha synuclein antibody pSerine 129; Alpha synuclein antibody pSer 129; Alpha synuclein antibody phosphoSer 129; Alpha-synuclein antibody; Alpha-synuclein; isoform NACP140 antibody; alphaSYN antibody; NACP antibody; Non A beta component of AD amyloid antibody; Non A4 component of amyloid antibody; Non A4 component of amyloid precursor antibody; Non-A beta component of AD amyloid antibody; Non-A-beta component of alzheimers disease amyloid; precursor of antibody; Non-A4 component of amyloid precursor antibody; Non-A4 component of amyloid; precursor of antibody; PARK 1 antibody; PARK 4 antibody; PARK1 antibody; PARK4 antibody; Parkinson disease (autosomal dominant; Lewy body) 4 antibody; Parkinson disease familial 1 antibody; SNCA antibody; alpha (non A4 component of amyloid precursor) antibody; SYN antibody; Synuclein alpha antibody; Synuclein alpha 140 antibody; Synuclein; alpha (non A4 component of amyloid precursor) antibody; SYUA_HUMAN antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human; Mouse
Purity/Purification
Peptide Affinity Purified
Specificity
Binds to phosphorylated serine 129 on alpha synuclein. Does not detect unphosphorylated serine 129 alpha synuclein. Detects bands at 100, 75, 45, 15 kDa. Bands above 15 kDa are oligomers.
Preparation and Storage
Store at -20 degree C.
Matching Pairs
Unconjugated Antibody: Alpha Synuclein (MBS8000787)
ATTO 488 Conjugated Antibody: Alpha Synuclein (MBS8000789)
ATTO 488 Conjugated Antibody: Alpha Synuclein (MBS8000789)
Matching Pairs
Unconjugated Antibody: N/A (MBS8000789)
ATTO 488 Conjugated Antibody: Alpha Synuclein (MBS8000789)
ATTO 488 Conjugated Antibody: Alpha Synuclein (MBS8000789)
Molecular Weight
13,109 Da
OMIM
127750
Target
Alpha Synuclein pSer129
Immunogen
Synthetic peptide of Human Alpha Synuclein pSer129 (40-140 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Immunogen Species
Human
Cellular Localization
Cytoplasm; Membrane; Nucleus; Cell Junction; Synapse; Secreted
Conjugate
ATTO 488
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Tissue Specificity
Highly expressed in brain (presynaptic nerve terminals) but is also expressed in low concentrations in all tissues examined except in liver.
Product Categories/Family for anti-SNCA antibody
Antibodies; Neuroscience; Neurodegeneration; Alzheimer's Disease; Tangles & Tau; Neuroscience; Neurodegeneration; Parkinson's Disease; Synuclein
Applications Tested/Suitable for anti-SNCA antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-SNCA antibody
WB: 1:1000
IHC: 1:500
IHC: 1:500
Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-SNCA antibody
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody (SPC-742). Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 ug/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody (SPC-742) at 1:500 for 24 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.
Western Blot (WB) of anti-SNCA antibody
Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa Alpha Synuclein pSer129 protein using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody (SPC-742). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody (SPC-742) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.
Product References and Citations for anti-SNCA antibody
1. "Genetics Home Reference: SNCA". US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757. 7. Fujiwara H., et al. (2002) Nat Cell Biol. 4(2):160-4. 8. Anderson J.P., et al. (2006) J Biol Chem. 281(40):29739-52.
Other Notes
Small volumes of anti-SNCA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SNCA purchase
MBS8000789 is an antibody targeting SNCA. Also known as Alpha Synuclein Antibody (pSer129): ATTO 488. Reactive in Human; Mouse. Host: Rabbit.
Additional Details
NCBI/Uniprot data below describe general gene information for SNCA. It may not necessarily be applicable to this product.
NCBI Official Full Name
alpha-synuclein isoform NACP140
NCBI Official Synonym Full Names
synuclein alpha
NCBI Official Symbol
SNCA
NCBI Official Synonym Symbols
PD1; NACP; PARK1; PARK4
UniProt Protein Name
Alpha-synuclein
UniProt Synonym Protein Names
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP
Protein Family
Alpha-synuclein
UniProt Gene Name
SNCA
UniProt Synonym Gene Names
NACP; PARK1; NACP
NCBI Protein Information
alpha-synuclein
NCBI Summary for SNCA
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]
Show more UniProt Comments for SNCA
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-SNCA antibody
| Reference Product | PubMed Publications |
|---|---|
| PARK2 antibody | >1228 publications with SNCA and PARK2 |
| PARK7 antibody | >67 publications with SNCA and PARK7 |
| SNCAIP antibody | >35 publications with SNCA and SNCAIP |
| UCHL1 antibody | >33 publications with SNCA and UCHL1 |
| APP antibody | >27 publications with SNCA and APP |
| SLC6A3 antibody | >16 publications with SNCA and SLC6A3 |
| FYN antibody | >4 publications with SNCA and FYN |
| KLK6 antibody | >1 publications with SNCA and KLK6 |
| SIAH2 antibody | >1 publications with SNCA and SIAH2 |
| UBB antibody | >1 publications with SNCA and UBB |
Pathways associated with anti-SNCA antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Alpha-synuclein Signaling Pathway antibodies | Alpha-synuclein Signaling Pathway Diagram |
| Alzheimer's Disease Pathway antibodies | Alzheimer's Disease Pathway Diagram |
| Alzheimer's Disease Pathway antibodies | Alzheimer's Disease Pathway Diagram |
| Alzheimers Disease Pathway antibodies | Alzheimers Disease Pathway Diagram |
| Amyloid Fiber Formation Pathway antibodies | Amyloid Fiber Formation Pathway Diagram |
| Ectoderm Differentiation Pathway antibodies | Ectoderm Differentiation Pathway Diagram |
| Metabolism Of Proteins Pathway antibodies | Metabolism Of Proteins Pathway Diagram |
| Parkin-Ubiquitin Proteasomal System Pathway antibodies | Parkin-Ubiquitin Proteasomal System Pathway Diagram |
| Parkinson's Disease Pathway antibodies | Parkinson's Disease Pathway Diagram |
| Parkinsons Disease Pathway antibodies | Parkinsons Disease Pathway Diagram |
Diseases associated with anti-SNCA antibody
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >1519 publications with SNCA and Nervous System Diseases |
| Brain Diseases Antibodies | >1496 publications with SNCA and Brain Diseases |
| Neurodegenerative Diseases Antibodies | >1472 publications with SNCA and Neurodegenerative Diseases |
| Movement Disorders Antibodies | >1321 publications with SNCA and Movement Disorders |
| Parkinsonian Disorders Antibodies | >1215 publications with SNCA and Parkinsonian Disorders |
| Parkinson Disease Antibodies | >1176 publications with SNCA and Parkinson Disease |
| Lewy Body Disease Antibodies | >336 publications with SNCA and Lewy Body Disease |
| Disease Models, Animal Antibodies | >219 publications with SNCA and Disease Models, Animal |
| Nerve Degeneration Antibodies | >182 publications with SNCA and Nerve Degeneration |
| PARKINSON DISEASE 1, AUTOSOMAL DOMINANT Antibodies | >70 publications with SNCA and PARKINSON DISEASE 1, AUTOSOMAL DOMINANT |
Organs/Tissues associated with anti-SNCA antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >1083 publications with SNCA and Brain |
| Nerve Antibodies | >1054 publications with SNCA and Nerve |
| Blood Antibodies | >138 publications with SNCA and Blood |
| Embryonic Tissue Antibodies | >29 publications with SNCA and Embryonic Tissue |
| Kidney Antibodies | >21 publications with SNCA and Kidney |
| Eye Antibodies | >19 publications with SNCA and Eye |
| Skin Antibodies | >15 publications with SNCA and Skin |
| Heart Antibodies | >15 publications with SNCA and Heart |
| Vascular Antibodies | >15 publications with SNCA and Vascular |
| Bone Antibodies | >11 publications with SNCA and Bone |