Anti-PRDM1/BLIMP1 (RABBIT) Antibody
Referencia ASR5483
embalaje : 100ug
Marca : Abcepta
Anti-PRDM1/BLIMP1 (RABBIT) Antibody
PRDM1 BLIMP1 Antibody
-
Immunohistochemistry of rabbit anti-PRDM1-BLMP1 antibody. Tissue: colon. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-PRDM1-BLMP1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: PRDM1-BLMP1 as precipitated red signal with hematoxylin purple nuclear counterstain.
Immunohistochemistry of rabbit anti-PRDM1-BLMP1 antibody. Tissue: colon. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-PRDM1-BLMP1 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: PRDM1-BLMP1 as precipitated red signal with hematoxylin purple nuclear counterstain.
| Host | Rabbit |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Human |
| Reactivity | Human |
| Clonality | Polyclonal |
Application 
| WB, IHC, E, IP, I, LCI |
| Application Note | This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~88 kDa in size corresponding to BLIMP1 by western blotting in the appropriate cell lysate or extract. |
| Physical State | Liquid (sterile filtered) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Anti-Blimp1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of human, mouse, and rat PRDM1/BLIMP1. |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 12142 |
|---|---|
| Other Names | 639 |
| Purity | This affinity purified antibody is directed against human BLIMP1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is predicted to react with BLIMP1 and its isoforms from human, mouse, rat and other sources based on a 100% homology with the immunizing sequence. This antibody may also recognize other proteins that show high homology/identity with the BLIMP1 sequence. |
| Storage Condition | Store Blimp1 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Prdm1 |
|---|---|
| Synonyms | Blimp1 |
| Function | Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs (PubMed:27102484). Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection (PubMed:27102484). Binds specifically to the PRDI element in the promoter of the beta-interferon gene (By similarity). Drives the maturation of B-lymphocytes into Ig secreting cells (By similarity). Associates with the transcriptional repressor ZNF683 to chromatin at gene promoter regions (PubMed:27102484). Binds to the promoter and acts as a transcriptional repressor of IRF8, thereby promotes transcription of osteoclast differentiation factors such as NFATC1 and EEIG1 (PubMed:32741026). |
| Cellular Location | Nucleus. Cytoplasm {ECO:0000250|UniProtKB:O75626} |
| Tissue Location | Expressed in bone marrow macrophages (at protein level) (PubMed:32741026). Expressed in innate lymphocytes, including tissue-resident conventional natural killer (cNK) cells in liver (PubMed:27102484). Expressed also weakly in tissue-resident natural killer (trNK) and natural killer T (NKT) cells in liver (PubMed:27102484). [Isoform 3]: Expressed only in the yolk sac. |
Research Areas
- Primary Antibodies
- Immunology
- Secondary Antibodies