Anti-SMRT Antibody (56217)
Referencia 56217-100
embalaje : 100ug
Marca : Leinco Technologies
AntiSMRT [Clone H7]
AntiSMRT [Clone H7]
Product No.: 56217
Clone H7 Target SMRT Formats AvailableView All Product Type Monoclonal Alternate Names NCoR2, CTG repeat protein 26, SMAP270, Silencing mediator of retinoic acid and thyroid hormone receptor, SMRT, T3 receptorassociating factor, TRAC, Thyroid, retinoicacidreceptorassociated corepressor Isotype Mouse IgG1 Applications ICC , IF , IP , WB |
Data
![HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDSPAGE, and the membrane was blotted with SMRT antibody [1542] (56217) diluted at 1:1000. The HRPconjugated antimouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plusEnhanced.](/proxy_img/aHR0cHM6Ly93d3cubGVpbmNvLmNvbS93cGNvbnRlbnQvdXBsb2Fkcy8yMDI1LzAxL3FlZGJpb3NjaWVuY2VhbnRpc21ydGFudGlib2R5NTYyMTcxLmpwZw==.jpg)
HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDSPAGE, and the membrane was blotted with SMRT antibody [1542] (56217) diluted at 1:1000. The HRPconjugated antimouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plusEnhanced.Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen A KLHconjugated synthetic peptide derived from human SMRT corresponding to aa 9941005 Product Concentration Lot Specific Formulation 10 mM PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by immunoaffinity chromatography Storage and Handling This antibody is stable for at least one (1) year at 20°C. Avoid multiple freeze thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 28°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting and Immunoprecipitation: use at 15 ug/mL. Positive control: HBL100 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes human SMRT. Background SMRT (Silencing Mediator for Retinoid and Thyroid receptors) is a 168 kD nuclear protein. Nuclear hormone receptors are transcription factors regulated by ligand binding. In the absence of ligand, there is a physical interaction between the nuclear receptors that do not interact with Hsp90 and a family of corepressors which includes SMRT. SMRT helps target histone deacetylases and other components of a multiprotein repressor complex to the specific target promoter to silence gene transcription. Function Transcriptional corepressor (PubMed:20812024). Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 4 have different affinities for different nuclear receptors. Involved in the regulation BCL6dependent of the germinal center (GC) reactions, mainly through the control of the GC Bcells proliferation and survival. Recruited by ZBTB7A to the androgen response elements/ARE on target genes, negatively regulates androgen receptor signaling and androgeninduced cell proliferation (PubMed:20812024). {PubMed:18212045, PubMed:20812024, PubMed:23911289}. NCBI Gene Bank ID UniProt.org Research Area Neuroscience References & Citations1. Iftode, C et al. 1999. Crit Rev Biochem Mol Biol 34: 141180 2. Bochkarev, A et al. 1999. EMBO 18: 44984504 3. Dong, J et al. 1999. Biochem J 337: 311317 4. Sibenaller, ZA et al. 1998. Biochemistry 37: 1249612506 5. Stigger, E et al. 1998. J Biol Chem 273: 93379343 6. Bochkareva, E et al. 1998. J Biol Chem 273: 39323936. |