Reactivos e instrumentos para inmunología, biología celular y biología molecular.
 
> Cholesterol [57-88-5]

Cholesterol [57-88-5]

Imprimir Imprimir
Por favor conéctese

Referencia NB-64-00922-10g

embalaje : 10g

Marca : Neo Biotech


Cholesterol (Synonyms: Cholesteryl alcohol, Cholesterin)

Catalog No. T0760 Copy Product Info
Purity: 99.94%
Cholesterol is the primary sterol in mammals, accounting for approximately 20–25% of the plasma membrane structure. It plays a key role in regulating membrane fluidity, permeability, and protein function. As an endogenous agonist of estrogen-related receptor α (ERRα), cholesterol is widely involved in metabolic regulation and serves as a precursor for the synthesis of hormones and bile acids. It is commonly used in experimental models of hyperlipidemia.

Cholesterol

Copy Product Info
Synonyms Cholesteryl alcohol, Cholesterin

Cholesterol is the primary sterol in mammals, accounting for approximately 20–25% of the plasma membrane structure. It plays a key role in regulating membrane fluidity, permeability, and protein function. As an endogenous agonist of estrogen-related receptor α (ERRα), cholesterol is widely involved in metabolic regulation and serves as a precursor for the synthesis of hormones and bile acids. It is commonly used in experimental models of hyperlipidemia.

Cholesterol
Cas No. 57-88-5
Other Forms of Cholesterol:

Batch Information
Select Batch
Purity:99.94%
Appearance:Solid
Color:White
Contact us for more batch information

Resource Download
COA LCMS HNMR

Product Introduction
Bioactivity
Description
Cholesterol is the primary sterol in mammals, accounting for approximately 20–25% of the plasma membrane structure. It plays a key role in regulating membrane fluidity, permeability, and protein function. As an endogenous agonist of estrogen-related receptor α (ERRα), cholesterol is widely involved in metabolic regulation and serves as a precursor for the synthesis of hormones and bile acids. It is commonly used in experimental models of hyperlipidemia.
In vitro
METHODS: CD4+ T lymphocytes were incubated with 7-KC (17.5-70 µM) and Cholesterol-MβCD (17.5-70 µM) for 10 min, and T cell membrane order and disorder were assessed using di-4 ANEPPDHQ fluorescent dye.
RESULTS: After exposure to 7-KC, T cell membrane order was altered in a dose-dependent manner, with significant reconstitution of membrane order observed only in cells treated with 35 µM Cholesterol, while reconstitution with l7.5 µM Cholesterol induced minimal effects. [1]
METHODS: Human gastric cancer cells SNU601, SNU638 and SNU216 were treated with Cholesterol (25-100 µM) for 48 h and cell viability was measured using MTT Assay.
RESULTS: Cholesterol caused a dose-dependent decrease in cell viability in all three cell lines. [2]
In vivo
METHODS: To induce hypercholesterolemia, STD:ddY mice were fed a high cholesterol diet (1% cholesterol, 0.5% cholic acid, 0.5% olive oil and 93% standard mouse chow).
RESULTS: Cholesterol can be used to construct a mouse model of hypercholesterolemia. [3]
METHODS: To induce hyperlipidemia, CD-1 mice were fed a high cholesterol diet (2% cholesterol and 0.6% sodium deoxycholate).
RESULTS: Cholesterol can be used to construct a mouse model of hyperlipidemia. [4]
Disease Modeling Protocol
Hyperlipidemia model
  • Modeling Mechanism：

    A high-cholesterol diet can increase serum cholesterol, induce lipid deposition in the aorta, and activate the inflammatory response (increased aortic cytokines), making it suitable for research on the inflammatory mechanisms of hyperlipidemia.

  • Related Products：

    Cholesterol (T0760)

  • Modeling Method：

    Experimental Subject：

    Guinea pigs, Hartley strain, Male, 8 weeks old, Body weight 300–350 g

    Dosage and Administration Route：

    ① Core modelling:
    - High-fat diet (0.5% cholesterol+5% butter);
    ② Control treatment: Control group administered cholesterol-free standard diet, all other conditions identical;

    Dosing Frequency and Duration Model：

    Ad libitum feeding, 10 weeks duration

  • Validation：

    1. Biochemical indicators: - Serum cholesterol: ≥5 mmol/L after 10 weeks of modeling (compared to 1.8 mmol/L in the control group); - Inflammatory factors: Serum TNF-α and IL-6 levels were significantly elevated; 2. Pathological indicators: - Aortic lipid deposition: Oil Red O staining showed intimal lipid aggregation, without obvious fibrous plaques; 3. Molecular indicators: - Upregulated expression of aortic inflammatory genes (VCAM-1, ICAM-1).

*Precautions：

*References：Xiangdong L,et,al. Animal models for the atherosclerosis research: a review. Protein Cell. 2011 Mar;2(3):189-201.

SynonymsCholesteryl alcohol, Cholesterin
Chemical Properties
Molecular Weight386.65
FormulaC27H46O
Cas No.57-88-5
SmilesC[C@@]12[C@]([C@]3([C@@]([C@]4(C)C(=CC3)C[C@@H](O)CC4)(CC1)07)07)(CC[C@@]2([C@@H](CCCC(C)C)C)07)07
Relative Density.1.06 g/cm3
Storage & Solubility Information
StorageShipping with blue ice/Shipping at ambient temperature.
Solubility Information
H2O: < 1 mg/mL (insoluble)
DMSO: < 1mg/mL (insoluble)
Ethanol: 11.42 mg/mL (29.54 mM), Sonication is recommended.
Solution Preparation Table
Ethanol
1mg5mg10mg50mg
1 mM2.5863 mL12.9316 mL25.8632 mL129.3159 mL
5 mM0.5173 mL2.5863 mL5.1726 mL25.8632 mL
10 mM0.2586 mL1.2932 mL2.5863 mL12.9316 mL
20 mM0.1293 mL0.6466 mL1.2932 mL6.4658 mL
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.