DBI Antibody
Referencia MBS9607944-0,2mL
embalaje : 0.2mL
Marca : MyBiosource
MBS9607944 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Western blot analysis of extracts of hela, using DBI antibody. The lane on the left is treated with the antigen-specific peptide.
DBI Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9607944This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid… More details
Product Overview
Online Datasheet in PDFProduct Name
DBI, Polyclonal Antibody
Full Product Name
DBI Antibody
Product Gene Name
anti-DBI antibody
Product Synonym Names
ACBD 1; ACBD1; ACBP; ACBP_HUMAN; Acyl CoA binding domain containing 1; Acyl CoA binding protein; Acyl Coenzyme A binding domain containing 1; Acyl coenzyme A binding protein; Acyl-CoA-binding protein; CCK RP; CCKRP; Cholecystokinin releasing peptide trypsin sensitive; DBI; Diazepam-binding inhibitor; Endozepine; EP; GABA receptor modulator; MGC70414
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Peptide affinity purification
Concentration
~1mg/ml (lot specific)
Specificity
DBI antibody detects endogenous levels of total DBI
Preparation and Storage
Store at -20°C. Stable for up to 12 months from date of receipt.
Antibody/Peptide Pairs
DBI peptide (MBS9626656) is used for blocking the activity of DBI antibody (MBS9607944)
Molecular Weight
10 kDa
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human DBI, corresponding to a region within the internal amino acids.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-DBI antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-DBI antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA (peptide): 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IHC: 1:50-1:200
ELISA (peptide): 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunohistochemistry (IHC) of anti-DBI antibody
MBS9607944 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Other Notes
Small volumes of anti-DBI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for DBI purchase
MBS9607944 is an antibody targeting DBI. Also known as DBI Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.
Additional Details
NCBI/Uniprot data below describe general gene information for DBI. It may not necessarily be applicable to this product.
NCBI Official Full Name
acyl-CoA-binding protein isoform 3
NCBI Official Synonym Full Names
diazepam binding inhibitor, acyl-CoA binding protein
NCBI Official Symbol
DBI
NCBI Official Synonym Symbols
EP; ACBP; ACBD1; CCK-RP
UniProt Protein Name
Acyl-CoA-binding protein
UniProt Synonym Protein Names
Diazepam-binding inhibitor; DBI; Endozepine; EP
Protein Family
Acyl-CoA-binding protein
UniProt Gene Name
DBI
UniProt Synonym Gene Names
ACBP; DBI; EP
NCBI Protein Information
acyl-CoA-binding protein
NCBI Summary for DBI
This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for DBI
Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters. It is also able to displace diazepam from the benzodiazepine (BZD) recognition site located on the GABA type A receptor. It is therefore possible that this protein also acts as a neuropeptide to modulate the action of the GABA receptor.
Show more Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-DBI antibody
| Reference Product | PubMed Publications |
|---|---|
| TSPO antibody | >7 publications with DBI and TSPO |
| CCK antibody | >5 publications with DBI and CCK |
| STAR antibody | >2 publications with DBI and STAR |
| PPARG antibody | >2 publications with DBI and PPARG |
Pathways associated with anti-DBI antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| PPAR Signaling Pathway antibodies | PPAR Signaling Pathway Diagram |
| PPAR Signaling Pathway antibodies | PPAR Signaling Pathway Diagram |
| SREBP Signalling Pathway antibodies | SREBP Signalling Pathway Diagram |
Diseases associated with anti-DBI antibody
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >78 publications with DBI and Nervous System Diseases |
| Disease Models, Animal Antibodies | >30 publications with DBI and Disease Models, Animal |
| Hemorrhage Antibodies | >15 publications with DBI and Hemorrhage |
| Liver Diseases Antibodies | >14 publications with DBI and Liver Diseases |
| Necrosis Antibodies | >8 publications with DBI and Necrosis |
| Neoplasms, Experimental Antibodies | >8 publications with DBI and Neoplasms, Experimental |
| Hepatic Encephalopathy Antibodies | >7 publications with DBI and Hepatic Encephalopathy |
| Edema Antibodies | >7 publications with DBI and Edema |
| Kidney Diseases Antibodies | >7 publications with DBI and Kidney Diseases |
| Carcinoma Antibodies | >7 publications with DBI and Carcinoma |
Organs/Tissues associated with anti-DBI antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >205 publications with DBI and Brain |
| Blood Antibodies | >98 publications with DBI and Blood |
| Nerve Antibodies | >35 publications with DBI and Nerve |
| Liver Antibodies | >34 publications with DBI and Liver |
| Vascular Antibodies | >31 publications with DBI and Vascular |
| Adrenal Gland Antibodies | >23 publications with DBI and Adrenal Gland |
| Kidney Antibodies | >19 publications with DBI and Kidney |
| Testis Antibodies | >19 publications with DBI and Testis |
| Heart Antibodies | >15 publications with DBI and Heart |
| Muscle Antibodies | >15 publications with DBI and Muscle |