gdhB Antibody (Biotin)

Referencia orb344306-100ug

embalaje : 100ug

Marca : Biorbyt


gdhB Antibody (Biotin)

SKU: orb344306

Description

Glucose Dehydrogenase antibody (Biotin)

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
ReactivityBacteria
Application Notes
Anti-Glucose Dehydrogenase has been assayed against 1.0 ug of Glucose Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 100 µL

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
IsotypeIgG
ImmunogenGlucose Dehydrogenase [Bacillus species]
TargetgdhB
PurityAnti-Glucose Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glucose Dehydrogenase [Bacillus species]. Cross reactivity against Glucose Dehydrogenase from other sources is unknown.
ConjugationBiotin

Storage & Handling

StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.0 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

goat anti-Glucose Dehydrogenase Antibody biotin Conjugation, biotin Conjugated goat anti-Glucose Dehydrogenase Antibody, gdh antibody, gdh2 antibody, gdh3 antibody, gdh4 antibody, gdhI antibody, GLCDHIII antibody, GLCDHIV antibody, Glucose 1 dehydrogenase 1 antibody
  • gdhB Antibody (Biotin) [orb344307]

    ELISA,  WB

    Bacteria

    Goat

    Polyclonal

    Biotin

    25 μl

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol