This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques (1/1,000-1/3,000).
Reactivity
Bovine
Antibody Host
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
Glutamate Dehydrogenase (Bovine Liver).
Specificity
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Buffer
PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized purified IgG fraction
Reconstitution Method
Restore by adding 1.0 ml of sterile distilled water.
Concentration
lot specific
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation
Unconjugated
Storage
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Avoid repeated freezing and thawing.
Glutamate dehydrogenase has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate. Glutamate, the main substrate of Glutamate dehydrogenase, is present in brain in concentrations higher than in other organs. In nervous tissue, Glutamate dehydrogenase appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
