Hsp40 (DNAJB1) (1-70) Mouse Monoclonal Antibody [Clone ID: 2E1]
CAT#: AM26593AF-N
Hsp40 (DNAJB1) (1-70) mouse monoclonal antibody, clone 2E1, Azide Free
Specifications
Product Data | |
Clone Name | 2E1 |
Applications | IP, WB |
Recommended Dilution | Western blot: 1-2 µg/ml for chemiluminescence detection system. Immunoprecipitation: 2-5 µg/200 µl of cell extract from 106 cells. For details see protocols below. |
Reactivities | Human, Mouse |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Full-length human HSP40 (1-340 aa) |
Specificity | This antibody recognizes J domain (1-70 aa) of hsp40. |
Formulation | PBS containing 50% glycerol, pH 7.2. No preservative is contained State: Azide Free State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein A agarose |
Conjugation | Unconjugated |
Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Gene Name | DnaJ heat shock protein family (Hsp40) member B1 |
Database Link | |
Background | 40 kDa heat shock protein (hsp40) is a novel heat shock protein induced by heat shock and other stresses in mammalian and avian cells. The cDNA sequence of the hsp40 is id entical with HDJ1. Deduced amino acid sequence of the hsp40 cDNA is homologues to DnaJ, an E. coli heat shock protein and its homologues in yeast such as SCJ1, YDJ1 (MAS5), SIS1, SEC63 and zuotin. E coli ’s DnaJ heat shock protein is known to function together with DnaK (hsp70) and GrpE as a molecular chaperone, which is necessary for assembly and disassembly of protein complexes, protein folding, renaturation of denatured proteins, prevention of protein aggregation and protein export. The hsp40 is colocarized with hsp70 in the nuclei and nucleoli of heat-shocked HeLa cells which suggests that hsp70 (DnaK)-hsp40 (DnaJ) chaperone system is ubiquitous. |
Synonyms | Heat shock 40 kDa protein 1, HSP-40, DnaJ protein homolog 1, HDJ-1, DNAJ1, HDJ1, HSPF1 |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting Immunoprecipitation |
Reference Data |
Documents
Product Manuals |
FAQs |
|
SDS |
Resources
Antibody Resources |
|