Quantibody® Human Angiogenesis Array 3 Kit. Detects 30 Human Angiogenic Factors. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 30 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Angiogenesis, Vascular Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| Angiopoietin-1
| Angiostatin
| CXCL16
| EGF
| FGF-4
|
| Follistatin
| GCSF
| GM-CSF
| I-309 (TCA-3/CCL1)
| IL-1 beta (IL-1 F2)
|
| IL-4
| IL-10
| IL-12 p40
| IL-12 p70
| I-TAC (CXCL11)
|
| MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
| MCP-4 (CCL13)
| MMP-1
| MMP-9
|
| PECAM-1 (CD31)
| TGF alpha
| TGF beta 3 | Tie-1
| Tie-2
|
| uPAR
| VEGF-A
| VEGFR2
| VEGFR3
| VEGF-D
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Angiogenesis Array Q3 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAH-ANG-3.gal)
Excel (QAH-ANG-3_Analysis_Tool.xls)
citations
Serum differentially expressed angiogenic cytokines in head and neck vascular malformations
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Journal of Oral Pathology & Medicine
Predictive value of angiogenic proteins in patients with metastatic melanoma treated with bevacizumab monotherapy
- Species:
- Human
- Sample Type:
- Serum (Biomarker of angiogenic proteins with metastatic melanoma and bevacizumab)
- Year:
- 2019
- PubMed ID:
- 30225999
(2014) Proangiogenic Compositions of Microvesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells
- Species:
- Human
- Sample Type:
- Cell Lysate (Microvesicles and MSC parents)
- Year:
- 2015
- Journal Name:
- Plos One
- PubMed ID:
- 25514634
Carbamylated low-density lipoprotein induces oxidative stress and accelerated senescence in human endothelial progenitor cells
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2011
- Journal Name:
- The FASEB Journal




