Orb392136-50ug | Human MGMT protein Clinisciences

Human MGMT protein

SKU: orb392136

Description

Recombinant human MGMT protein is produced by our E. coli expression system and the target gene encoding Met1-Asn207 is expressed with a 6His tag at the N-terminus.

Research Area

Epigenetics & Chromatin

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	E. coli
Target	MGMT
Reactivity	Human
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Protein Sequence	MGSSHHHHHH SSGLVPRGSH MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPALHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN

Storage & Handling

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Storage	Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.2 μM filtered solution of 20mM Tris-HCl, 1mM EDTA, pH 8.0.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

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Human O-6-Methylguanine DNA Methyltransferase (MGMT) ELISA Kit [orb775646]
Reactivity:
Human
Dynamic Range:
0.16-10 ng/mL
Sensitivity:
0.053 ng/mL
Sizes Available: 48 T, 96 T
Human O-6-Methylguanine DNA Methyltransferase (MGMT) ELISA Kit [orb1948247]
Reactivity:
Human
Dynamic Range:
0.16-10ng/mL
Sensitivity:
0.1 ng/mL
Sizes Available: 48 T, 96 T
Rabbit Human MGMT Antibody [orb109557]
Applications:
ELISA, IHC-P, WB
Reactivity:
Human
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μg
MGMT Mouse Monoclonal Antibody [orb2988236]
Applications:
FC, IF, IHC, WB
Reactivity:
Human
Species/Host:
Mouse
Clonality:
Monoclonal
Conjugation:
Unconjugated
Sizes Available: 200 μl, 100 μl, 50 μl, 30 μl
MGMT Mouse Monoclonal Antibody [orb1473978]
Applications:
IF, IHC, WB
Reactivity:
Human
Species/Host:
Mouse
Clonality:
Monoclonal
Conjugation:
Unconjugated
Sizes Available: 200 μl, 100 μl, 50 μl, 30 μl