Labeled human holo-transferrin is used for microscopic studies of the endosomal pathway and/or transferrin uptake. Biotium’s holo-transferrin conjugates are labeled with a selection our CF™ dyes, a series of next-generation fluorescent dyes developed at Biotium to have combined advantages in brightness, photostability, and water solubility compared to other fluorescent dyes.

• Choice of 7 CF® dyes from green to near-infrared
• Superior CF® dyes are bright, photostable, and water-soluble
• For endocytosis and transferrin uptake studies

Transferrin is a glycoprotein with homologous N-terminal and C-terminal iron-binding domains that binds up to two Fe(III) atoms for delivery to vertebrate cells through receptor-mediated endocytosis. After binding to its receptor on the cell surface, transferrin is rapidly internalized by invagination of clathrin-coated pits with formation of endocytic vesicles, the acidic environment favors dissociation of iron from the transferrin-receptor complex. Following the release of iron, the transferrin is then recycled back to the surface of the cell via the endocytic recycling pathway and released to bind more iron.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

CF® Dye Human Transferrin