MHC Class I (PE)

Recognizes the ovine homologue of the human MHC Class I, a monomorphic determinant expressed on the heavy chain of sheep MHC Class I, (OLA Class I).

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In sheep, this is often referred to as the ovine leukocyte antigen (OLA) region. Ovine MHC Class I functions in the recognition and presentation of foreign antigens to T-cells.

Ovine MHC Class I is a membrane glycoprotein with a molecular weight of ~44kD, expressed on the cell surface of all peripheral blood leucocytes.

Has been in used in studies on a number of domestic animal diseases, in particular Maedi Visna virus infection, a disease of significant inportance in commercial sheep flocks (Lee et al. 1996, Ryan et. al. 2000 and Wu et. al. 2008). Has been cited as recognising MHC class I in some other species and has been used in a study of Feline herpes virus infection (Montagnaro et. al. 2009).

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:25-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.