Mouse Human IgG2 (Fc subclass specific), conjugated with TRITC Antibody

Referencia orb22266-0.2mg

embalaje : 0.2mg

Marca : Biorbyt


Mouse Human IgG2 (Fc subclass specific), conjugated with TRITC Antibody

SKU: orb22266

Description

Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgG2, subclass specific. To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50; in ELISA from 1:200 upwards; in Western blotting from 1:400 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Images & Validation

ReactivityHuman

Key Properties

Antibody TypeSecondary Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.NI 25-1 (HP 6207)
ConjugationTRITC

Storage & Handling

StorageThe lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.
Form/AppearancePurified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only
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