Mouse TNF Alpha Protein (Recombinant)
Referencia LS-G137545-10
embalaje : 10ug
Marca : LSBio
Overview
Description:
TNF Alpha Protein LS-G137545 is a Recombinant Mouse TNF Alpha. It is low in endotoxin; Less than 0.01 ng/µg (Less than 0.1 EU/µg) (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Species
Mouse
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 156 amino acid recombinant protein has a predicted molecular mass of 17,257 Da. The DTT-reduced and non-reduced protein migrate at approximately 16 kD by SDS-PAGE. The N-terminal amino acid is Leu.
Source Species
E. coli
Purification
Greater than 98% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA.
Bio-Activity
Cytotoxic effect on L929 mouse fibroblast cells induced by mouse TNF-α in the presence of actinomycin D. The ED50 for this effect is “ 3 pg/mL.
Endotoxin
Less than 0.01 ng/µg (Less than 0.1 EU/µg) (determined by LAL method).
Presentation
0.22 µm filtered protein solution, pH 7.2, 10 mM NaH2PO4, 150 mM NaCl
Storage
Store unopened at 2°C to 8°C for up to 1 month, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee About TNF Alpha
P01375 NM_000594 NP_000585.2
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
tech@clinisciences.com