MSX1 Blocking Peptide

Formulation:	Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	MSX1
Gene ID:	4487
Uniprot ID:	MSX1_HUMAN
Immunogen Region:	46-64
Specificity:	This blocking peptide is recommended for use in combination with MSX1 antibody, STJ501812
Immunogen:	Synthetic peptide: DEEGAKPKVSPSLLPFSVE corresponding to amino acids 46-64 of Mouse Msx1 protein. The peptide was post synthetically modified and conjugated to KLH using hetero bifunctional cross linker for immunization of rabbits.

Post Translational Modifications	Sumoylated by PIAS1, desumoylated by SENP1. Sumoylation of Lys-15 and Lys-133 not required for interaction with H1-5, transcriptional repression, inhibition of myoblast differentiation, or binding to gene promoters.
Function	Acts as a transcriptional repressor. Capable of transcription autoinactivation. Binds to the consensus sequence 5'-C/GTAAT-3' in downstream activin regulatory elements (DARE) in the gene promoter, thereby repressing the transcription of CGA/alpha-GSU and GNRHR. Represses transcription of myoblast differentiation factors. Binds to core enhancer regions in target gene promoters of myoblast differentiation factors with binding specificity facilitated by interaction with PIAS1. Recruits histone H3 methyltransferases such as EHMT2/G9a to gene promoter regions which leads to inhibition of myoblast differentiation via transcriptional repression of differentiation factors. Regulates, in a stage-specific manner, a developmental program of gene expression in the fetal tooth bud that controls odontoblast differentiation and proliferation of dental mesenchymal cells. At the bud stage, required for mesenchymal molar tooth bud development via facilitating reciprocal signaling between dental epithelial and mesenchymal cells. May also regulate expression of Wnt antagonists such as DKK2 and SFPR2 in the developing tooth mesenchyme. Required for BMP4 expression in dental mesenchyme cells. Also, in response to BMP4, required for BMP4 expression in neighboring dental epithelial cells. Required for maximal FGF4-induced expression of SDC1 in dental mesenchyme cells. Also in response to SDC1, required for SDC1 expression in neighboring dental epithelial cells. At the early bell stage, acts to drive proliferation of dental mesenchyme cells, however during the late bell stage acts as an homeostatic regulator of the cell cycle. Regulates proliferation and inhibits premature mesenchymal odontogenesis during the bell stage via inhibition of the Wnt signaling component CTNNB1 and subsequent repression of the odontoblast differentiation factors BMP2, BMP4, LEF1, ALPL and BGLAP/OCN. Additionally, required for correct development and fusion of the palatal shelves and embryonic mandibular formation. Plays a role in embryonic bone formation of the middle ear, skull and nasal bones. Required for correct formation and thickness of the nail plate. May play a role in limb-pattern formation.
Peptide Name	Homeobox Protein Msx-1 Homeobox Protein Hox-7 Msh Homeobox 1-Like Protein
Cellular Localisation	Nucleus Interaction With Ehmt2/G9a Is Required For Localization To The Nuclear Periphery Interaction With Pias1 Is Required For Localization To The Nuclear Periphery
Alternative Peptide Names	Homeobox Protein Msx-1 protein Homeobox Protein Hox-7 protein Msh Homeobox 1-Like Protein protein MSX1 protein HOX7 protein

Information sourced from Uniprot.org

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