MUC5AC ELISA Kit (Mouse)
Referencia OKDD00616
embalaje : 96T
Marca : Aviva Systems Biology
MUC5AC ELISA Kit (Mouse) (OKDD00616)
| Datasheets/Manuals | Printable datasheet for MUC5AC ELISA Kit (Mouse) (OKDD00616) |
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| Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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| Application | ELISA | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology MUC5AC ELISA Kit (Mouse) (OKDD00616) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for MUC5AC has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MUC5AC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MUC5AC captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively. Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate. Mean Intra-Assay: CV<10% Mean Inter-Assay: CV<12% | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell lysates | ||||||||||||||||||||||
| Sensitivity | <0.053 ng/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of MUC5AC. No significant cross-reactivity or interference between MUC5AC and analogues was observed. | ||||||||||||||||||||||
| Assay Info | Quantitative Sandwich ELISA |
| Gene Symbol | Muc5ac |
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| Gene Full Name | mucin 5, subtypes A and C, tracheobronchial/gastric |
| Alias Symbols | 2210005L13Rik;gastric mucin;MGM;mucin-5AC. |
| NCBI Gene Id | 17833 |
| Protein Name | Mucin 5, subtypes A and C, tracheobronchial/gastric |
| Uniprot ID | E9PWB6 |
| Protein Accession # | NP_034974.1 |
| Nucleotide Accession # | NM_010844.1 |
| Protein Size (# AA) | 2751 |
| Molecular Weight | 299 kDa |
