NeoFluor™ dsDNA Quantification Kit

Description

NeoFluor dsDNA Kit from Neo Biotech enables accurate and sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in purified samples. It is suitable for use with Qubit™ fluorometers (Thermo Fisher Scientific) or similar instruments.

The kit contains a proprietary fluorescent DNA-binding dye with excitation at 488 nm and emission at 522 nm.

The assay is highly selective for dsDNA over RNA and provides a linear range of 0.2–200 ng of dsDNA input, corresponding to sample concentrations of 10 pg/µL to 100 ng/µL.

Compared with spectrophotometric DNA quantitation, it offers significantly increased sensitivity, high selectivity for dsDNA over single-stranded DNA (ssDNA) or RNA, and improved tolerance to contaminants such as proteins and carbohydrates.

The kit is ideal for quantifying DNA from low-concentration or precious samples, and for preparing DNA for sensitive downstream applications such as next-generation sequencing (NGS) and digital PCR.

Key Benefits

• High sensitivity: accurate quantitation of small amounts of dsDNA.
• High selectivity: preferential detection of dsDNA over ssDNA or RNA.
• Improved contaminant tolerance: greater tolerance to protein and carbohydrate contamination than spectrophotometric DNA quantitation.
• Suitable for demanding applications: DNA quantitation for NGS and digital PCR, including low-concentration or precious samples.

Product Properties