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> Nutrient Mixture Ham's F-12 w/L-Glutamine (Powder)

Nutrient Mixture Ham's F-12 w/L-Glutamine (Powder)

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Referencia MBS652910-10L

embalaje : 10L

Marca : MyBiosource


Nutrient Mixture Ham's F-12 w/L-Glutamine Culture Media

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS652910

Ham's Nutrient Mixtures were originally developed to support clonal growth of several clones of Chinese hamster ovary (CHO) cells, as well as clones of HeLa and mouse L-cells. Both mixtures were formulated for use with or without serum supplementation,… More details

Product Overview

Product Name
Nutrient Mixture Ham's F-12 w/L-Glutamine, Culture Media
Full Product Name
Nutrient Mixture Ham's F-12 w/L-Glutamine (Powder)
Purity/Purification
Cell Culture Grade
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Endotoxin Level
1EU/ml
Appearance
Light pink, homogenous, free flowing powder
pH
8.67
Solubility
Pink, clear, complete
Directions per Liter
Dissolve 10.70 g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 1.18g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Components shown as g/L
Inorganic Salts
Calcium Chloride•2H2O - 0.0441
Cupric Sulfate•5H2O - 0.0000025
Ferrous Sulfate•7H2O - 0.000834
Magnesium Chloride•6H2O - 0.123
Potassium Chloride - 0.224
Sodium Chloride - 7.599
Sodium Phosphate Dibasic - 0.14204
Zinc Sulfate•7H2O - 0.000863

Amino Acids
L-Alanine - 0.009
L-Arginine•HCl - 0.211
L-Asparagine•H2O - 0.01501
L-Aspartic Acid - 0.0133
L-Cysteine•HCL•H2O - 0.035
L-Glutamic Acid - 0.0147
L-Glutamine - 0.146
Glycine - 0.00751
L-Histidine•HCl•H2O - 0.02096
L-Isoleucine - 0.00394
L-Leucine - 0.0131
L-Lysine•HCl - 0.0365
L-Methionine - 0.00448
L-Phenylalanine - 0.00496
L-Proline - 0.0345
L-Serine - 0.0105
L-Threonine - 0.0119
L-Tryptophan - 0.00204
L-Tyrosine•Na2•2H2O - 0.00778
L-Valine - 0.0117

Vitamins
D-Biotin - 0.0000073
Choline Chloride - 0.01396
Vitamin B-12 - 0.00136
Folic Acid - 0.00132
myo-Inositol - 0.018
Niacinamide - 0.000037
D-Pantothenic Acid•Ca - 0.00048
Pyridoxine•HCl - 0.000062
Riboflavin - 0.000038
Thiamine•HCl - 0.00034

Other
Thymidine - 0.00073
D-Glucose - 1.802
Hypoxanthine - 0.00408
Linoleic Acid - 0.000084
Thioctic Acid - 0.00021
Phenol Red, Sodium - 0.0013
Putrescine•2HCl - 0.000161
Pyruvic Acid, Sodium - 0.11

Total: 10.702g/liter

Product Categories/Family for Nutrient Mixture Ham's F-12 w/L-Glutamine culture media

Culture Media; Media-Cell Culture; Nutrient Mixture Ham's

Other Notes

Small volumes of Nutrient Mixture Ham's F-12 w/L-Glutamine culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Nutrient Mixture Ham's F-12 w/L-Glutamine purchase

MBS652910 is a culture media targeting Nutrient Mixture Ham's F-12 w/L-Glutamine. Also known as Nutrient Mixture Ham's F-12 w/L-Glutamine (Powder).

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