Protein Molecular Weight Marker

Referencia orb1567188-10x200ul

embalaje : 10x200ul

Marca : Biorbyt


Protein Molecular Weight Marker

SKU: orb1567188

Description

Protein Molecular Weight Marker

Images & Validation

Application Notes
Protein Molecular Weight Marker has been tested and is designed for use in accurate estimation of protein molecular weight in SDS-polyacrylamide gel electrophoresis and western blotting. Thaw the cocktail at room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 5-10µl is appropriate for most gel formats

Key Properties

PurityProtein Molecular Weight Marker is supplied in 1X loading buffer and is ready-to-use. It contains: [62.5 mM Tris-HCl, pH 6.8, 2% (w/v) SDS, 250 mM NaCl, 1mM EDTA, 4% (v/v) β-Mercaptoethanol, 35% (v/v) Glycerol and 0.05% (w/v) bromophenol blue.] This product are unstained markers optimized for use with Coomassie blue staining, but the loading volume can be adjusted for compatibility with other staining methods (i.e. silver staining). The intensity of the 67 kDa bovine albumin band has been increased to serve as a reference.
ConjugationUnconjugated

Storage & Handling

StorageStore vial at -20° C.
Form/AppearanceLiquid (in 1x Loading Buffer)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 2.5 mM Tris-HCl, pH 6.8, 2% (w/v) SDS, 250 mM NaCl, 1mM EDTA, 4% (v/v) β-Mercaptoethanol, 35% (v/v) Glycerol and 0.05% (w/v) bromophenol blue
Concentration1X
Expiration Date6 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

Protein ladder, Protein marker, Protein standard, Protein molecular weight standard, Molecular weight marker, Protein molecular weight ladder, Protein marker, Mw marker, Mw standard
Protein Molecular Weight Marker

SDS PAGE of Protein Molecular Weight Marker, 14-120 kDa

Protein Molecular Weight Marker

SDS PAGE Results of Protein Molecular Weight Marker 14-120kDa. Lane 1: Opal Prestained Molecular Weight Marker (11-245kDa). [7 µl] Lane 2: Protein Molecular Weight Marker (14-120kDa) (p/n orb348606). [5 µl] 4-20% Gel, Coomassie Stained.

Protein Molecular Weight Marker

SDS-Page of Protein Molecular Weight Marker. Lane 1: Protein Molecular Weight Marker 12.5 uL. Lane 2: Protein Molecular Weight Marker 10.0 uL. Lane 3: Protein Molecular Weight Marker 7.5 uL. Lane 4: Protein Molecular Weight Marker 5.0 uL. Exact molecular weights are detailed on image 1.

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Protocol Information

SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
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Protein Molecular Weight Marker (orb1567188)

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