Orb775023-48Tests | Rat Interleukin 6 (IL6) ELISA Kit Clinisciences

Rat Interleukin 6 (IL6) ELISA Kit

SKU: orb775023

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Description

The Rat Interleukin 6 (IL6) ELISA Kit uses a sandwich enzyme immunoassay to quantify Interleukin 6 (IL6) in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 7.82-500 pg/mL and a sensitivity of 3.3 pg/mL.

Research Area

Immunology & Inflammation

Images & Validation

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Tested Applications	ELISA
Application Notes	standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL6 in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Application Notes

standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL6 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	7.82-500 pg/mL
Sensitivity	3.3 pg/mL
Concentration	500 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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MGI2-A ELISA Kit, MGI2A ELISA Kit, HGF ELISA Kit, BSF2 ELISA Kit, HSF ELISA Kit, IFNB2 ELISA Kit, B-Cell Stimulatory Factor-2 ELISA Kit, Hybridoma/Plasmacytoma Growth Factor ELISA Kit, Hepatocyte Stimulating Factor ELISA Kit, Cytotoxic T-Cell Differentiation Factor ELISA Kit

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Rat Interleukin 6 (IL-6) High Sensitivity ELISA Kit [orb1945921]
Reactivity:
Rat
Dynamic Range:
1.56-100pg/mL
Sensitivity:
0.94 pg/mL
Sizes Available: 48 T, 96 T
Rat Interleukin 6 (IL-6) Microsample Fast ELISA Kit [orb1806741]
Reactivity:
Rat
Dynamic Range:
12.5-800pg/mL
Sensitivity:
7.5 pg/mL
Sizes Available: 48 T, 96 T
Rat Interleukin 6 (IL-6) ELISA Kit [orb1806966]
Reactivity:
Rat
Dynamic Range:
31.25-2000pg/mL
Sensitivity:
28.77 pg/mL
Sizes Available: 48 T, 96 T
Rat IL-6 ELISA Kit [orb1085987]
Reactivity:
Rat
Dynamic Range:
62.5 - 4000 pg/mL
Sensitivity:
32 pg/mL
Sizes Available: 96 T
Rat IL6 ELISA Kit [orb410737]
Reactivity:
Rat
Dynamic Range:
0.312 pg/ml-20 pg/ml
Sensitivity:
0.078 pg/ml
Sizes Available: 48 T, 96 T, 5 x 96 T, 10 x 96 T, 24 T